TL;DR
This Primer provides a comprehensive guide to the experimental IMC workflow, covering antibody conjugation and panel design, sample preparation, data acquisition and quality control, and detail the computational analysis pipeline, including image preprocessing, segmentation, feature extraction, and both single-cell and spatial analyses.
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been established as a powerful tool for the spatial characterization of molecules. Recent advances have been driven by improvements in the spatial resolution, acquisition time, and sensitivity. Although MALDI-MSI excels in the label...
Imaging flow cytometry (IFC), an instrument that combines the features of microscopy with image acquisition and microfluidic chips, enables the rapid analysis of single-cell image information at high throughput. Currently, imaging flow cytometers have been integrated with deep learning for classification purposes. Howe...
Same-tissue section multimodal imaging is a powerful strategy that spatially profiles tissue histology, cell populations and molecular composition while maximizing tissue economy, preserving spatial molecular relationships, and increasing co-registration capacity. However, performing multiple modalities on the same tis...
Fluorescence lifetime imaging microscopy (FLIM) is an advanced microscopy technique that enables label-free, non-destructive optical metabolic imaging (OMI) of biological materials at spatiotemporal resolution. Accordingly, FLIM has emerged as a widely used platform for multi-scale characterization of the tumor mic...
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