Aug 2026· Stress Biology· Vol 6· 0 citations· 54 references
Medicine
TL;DR
This study elucidates the regulation of APA mediated by SIZ1 during HS response and establishes a strategy for identifying specific transcripts arising from APA for plant heat tolerance.
Abstract
Heat stress (HS) has emerged as a significant environmental factor affecting plant growth and agricultural productivity. Alternative polyadenylation (APA) is a crucial co-transcriptional process that regulates developmental processes and stress responses in plants. However, the distinct roles of its resultant transcripts in plant HS response remain to be investigated. In this study, we employed poly(A) tag sequencing (PAT-seq) to identify over 1500 transcripts whose expression exhibited significant alterations in response to HS, mediated by SIZ1, a SUMO E3 ligase in Arabidopsis. Further analysis revealed that more than 300 switch genes with varying poly(A) site usages were differentially expressed under HS conditions. Compared to non-canonical poly(A) sites, more genes utilize 3’UTR sites to regulate transcript expression by altering the usage of poly(A) signals. Based on the information of APA genes regulated by SIZ1, we selected two genes, Galactinol synthase enzyme (GolS2) and Tetratricopeptide repeat (TPR) like 3 (TTL3), for characterizing their novel functions. Overexpression of the distal transcript of GolS2 or the proximal transcript of TTL3 enhanced heat tolerance in Arabidopsis. Collectively, our current study elucidates the regulation of APA mediated by SIZ1 during HS response and establishes a strategy for identifying specific transcripts arising from APA for plant heat tolerance.
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Understanding of how ZmJAZ genes contribute to drought adaptation in maize is advanced, offering a conceptual framework that links molecular responses to climate-resilient traits essential for sustainable crop production under water-limited conditions in the face of climate change.
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Analysis of OsHSF genes in the japonica and indica rice cultivars offered valuable insights into the function of OsHSF genes that will contribute to the development of climate-resilient rice cultivars, as indicated by gene structure analysis.
Almas Danish, Muhammad Saeed, Pingfang Yang· International Journal of Mol...· 0 citations
Coffee is a livelihood source for millions of farmers and plays a significant role in the economy of many coffee-producing countries. Abiotic stresses, such as drought and high temperature, however, negatively impact coffee growth, productivity, and bean quality. Heat shock factors (HSFs) play a crucial role in the plant response to heat and other abiotic stresses. However, there is still not a lot of detailed knowledge about the HSF gene family in Coffea arabica. In this study, a genome-wide search was performed to find and characterize the genes of HSF from C.arabica. A total of 59 putative CaHSF genes have been identified and characterized according to their physicochemical properties, chromosomal distribution, gene structure, conserved motifs, phylogenetic relationship, duplication event, synteny, cis-regulatory elements, and protein–protein interactions. The genes identified for CaHSF were assigned to different scaffolds of the genome, and they had different sizes, molecular mass, isoelectric point, GRAVY value, and aliphatic index of their protein. Through the relationships induced by the phylogenetic and conserved-domain analyses, and by their conservation, it was possible to get insight into the evolution of the proteins of the CaHSF family. Abiotic stress response, hormone signaling, and light regulation were noted to have several cis-regulatory elements associated with them in the promoter analysis and hence the possible role of the CaHSF genes in stress adaptation. Duplication and synteny analyses also showed that gene duplication played a role in the diversification and expansion of the CaHSF gene family in C. arabica. Overall, this study is a thorough genomic analysis of the CaHSF gene family, which can serve as a basis for future functional studies for understanding the molecular mechanism of abiotic stress tolerance in coffee.
S. Naeem, Z. Ali, Z. Naeem et al.· Bulletin of Biological and A...· 0 citations
An extensive genome-wide study of the DUF668 gene family in potato demonstrated that StDUF668s play a role in crucial biological processes, involving abiotic and biotic stress responses, and provided a valuable resource for future research aimed at improving potato stress tolerance and growth.
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Together, these findings provide a foundation for functional characterization and useful information for future research on the role of SlPHD family members in plant abiotic stress tolerance.
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