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Isolation of Primary RNA for Differential RNA Sequencing in Fusobacterium nucleatum.

2026 · Methods in molecular biology · Vol 3055, pp. 109-114 · 0 citations
Medicine

TL;DR

This protocol enables effective cell lysis, nucleic acid purification, and efficient removal of genomic DNA while preserving all transcript classes, including small regulatory RNAs (sRNAs), and provides a basis for future studies investigating the gene expression dynamics and regulatory networks of F. nucleatum under diverse environmental conditions.

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