A baseline chromatin-state landscape in reef-building corals is defined, offering a resource for validating targeted chromatin assays and a foundation for future studies of coral gene regulation across environmental, developmental, and symbiotic contexts.
RNA-binding proteins (RBPs) are increasingly recognized to perform chromatin-associated functions in addition to their canonical roles in RNA regulation, yet how these activities are coordinated in human embryonic stem cells (hESCs) remain poorly understood. Here, we characterize distinct RNA- and chromatin-associated...
The findings uncover a non-canonical mechanism whereby a chromatin remodeler regulates transcription primarily through three-dimensional genome organization rather than local accessibility control, and establish histone modification-guided chromatin remodeling as a key principle in gene regulation.
Ming Yu, Jingdong Xue, Qi Zhang et al.· bioRxiv· 0 citations
The six-residue loop 2 of H2A and H2A is used to map, at single-residue resolution, how nucleosome-core variation reshapes cellular function, and identifies histone variation as a source of phenotypic innovation.
Zachary H. Harvey, Benjamin Gundinger, Jian-Yi Kok et al.· bioRxiv· 0 citations
Dynamic histone modifications are critical for regulating stem cell fate. However, generalized strategies for site-specific programming of histone modifications profiling on unperturbed chromatin in embryonic stem cells (ESCs) remain challenging. In this study, we established a genetic code expansion-based platform in...
Fang-Ni Chai, Qin Huang, Li Zhou et al.· Nature Communications· 0 citations
Transcription and RNA processing are tightly coupled to chromatin organization, yet how nucleosome positioning is coordinated across the promoter and gene body to shape alternative isoform expression remains poorly understood. This question has been difficult to address because short-read chromatin profiling cannot sim...
HisTrader is presented, a computational approach that identifies nucleosome-free regions (NFRs) within ChIP-based profiling of histone modification peaks, which reduces the target sequence length for motif discovery and genetic variant prioritization.