Aug 2026· Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy· Vol 364 Pt 2, pp.
128610
· 0 citations· 65 references
Medicine
TL;DR
A dual-functional iridium complex-based fluorescent probe Ir-NO2 is designed and synthesized that targets mitochondria, specifically recognizes NTR, and serves as an NTR-activatable photosensitizer for photodynamic therapy (PDT) of tumor cells.
Abstract
Nitroreductase (NTR) can efficiently reduce the nitro group of compounds to an amino group using nicotinamide adenine dinucleotide (NADH) as an electron source. Its expression is upregulated under hypoxic conditions, making it a reliable marker for the degree of hypoxia in tumor cells. Herein, we designed and synthesized a dual-functional iridium complex-based fluorescent probe Ir-NO2 that targets mitochondria, specifically recognizes NTR, and serves as an NTR-activatable photosensitizer for photodynamic therapy (PDT) of tumor cells. The probe is capable of detecting both endogenous and exogenous NTR in cells, as well as enabling NTR recognition in zebrafish. Upon NTR detection, the singlet oxygen yield increased from 0.71 to 0.83. Moreover, both the probe and its NTR-recognized strongly fluorescent produce (Ir-OH) function as dual Type I/II PDT photosensitizers.
Irpiq-DNP enabled longitudinal imaging of Sec fluctuations in dextran sulfate sodium (DSS)-induced colitis mice, where the luminescence intensity closely correlated with disease progression as well as therapeutic response to 5-aminosalicylic acid (5-ASA) treatment.
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