TL;DR
This chapter presents protocols for high-throughput, sequence-based analysis of AMF communities, covering the complete workflow from DNA extraction in plant or soil samples to the bioinformatic processing of sequencing data.
16S rDNA acts as a genetic fingerprint, helping scientists classify and track the evolutionary history of bacteria. Modern rapid sequencing tools, like Illumina, leverage the sequence differences (variation) in this gene to quickly identify all the bacterial species within an environmental sample, making this an import...
Adequate naming of medically significant plant species is crucial to the conservation of biodiversity, taxonomic verification and contaminating mixtures of herbs. The current research was intended to molecularly verify Rivina humilis with the help of chloroplast DNA barcoding. Genomic DNA was amplified of the chloropla...
Members of the genus Pantoea are known for their high ecological plasticity and diverse interactions with plants, ranging from phytopathogenic to commensal, symbiotic, and growth-promoting. Some of its strains have significant biotechnological potential due to their ability to synthesize phytohormones, siderophores, an...
Antarctic soils harbor highly specialized bacterial communities that perform essential functions in the functioning of polar ecosystems; however, the composition and structure of these communities remain poorly understood in many areas of Antarctica. The objective of this study was to characterize the diversity and str...
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