Aug 2026· Journal of Agricultural and Food Chemistry· Vol 74 32, pp.
25463-25475
· 1 citation· 67 references
Medicine
Abstract
Fruit pomace recycling is critical for green biomanufacturing. Lactococcus lactis, a common host for waste valorization, shows poor protein expression in conventional systems. We constructed T7RNAP-driven inducible and constitutive expression systems in L. lactis NZ9000. The two systems increased target gene transcription by 103-fold and 207-fold, and enzyme activity by 5.24-fold and 10.36-fold, respectively. A signal peptide enabled efficient extracellular secretion of recombinant proteins. The engineered strains synthesized levansucrase and levan, achieving yields of 36.27 g/L and 27.53 g/L in sucrose medium. When using food waste substrates, the levan titer reached 8.84 g/L with molasses, and 3.2, 1.4, 0.5, 0.7, 0.5 g/L with sugar cane bagasse and various fruit peels. This work provides a reliable strategy for strain engineering, pomace reuse and low-cost large-scale levan production.
Lactosucrose is a functional trisaccharide with prebiotic properties and growing potential in food and agricultural applications; however, its industrial production is limited by low conversion efficiency and high downstream processing costs. In this study, a whole-cell biocatalyst was developed by displaying β-galacto...
Indigoidine is a sustainable natural blue pigment with broad industrial prospects, while its microbial production is hampered by growth inhibition of the producing strain, enzyme-host incompatibility, and insufficient industrial-scale titer. In this study, de novo biosynthesis of indigoidine was achieved in Corynebac...
Jun-Jie Sun, Hui Gao, Xin-Yang Xu et al.· Journal of Agricultural and...· 0 citations
A multiomics framework for LSZ23-mediated lignocellulose bioconversion is provided and it is identified as a candidate hydrolytic partner for straw degrading consortia.
Wei Zhang, Gong-Fu Shi, Wen-Qing Cao et al.· Frontiers in Microbiology· 0 citations
Introduction Laccases are versatile oxidoreductases found in bacteria, fungi, and plants., which have a wide range of industrial applications, including the removal of pharmaceutical micropollutants from wastewater. Bacterial laccases are particularly attractive because of their high catalytic activity and stability ov...
Henrik Nausch, C. Bernau, Dirk Scheffler et al.· Frontiers in Plant Science· 0 citations
Lacto-N-tetraose (LNT), a core component of human milk oligosaccharides (HMOs), plays essential biological roles; however, its production remains challenging due to low conversion efficiency and the accumulation of intermediates. In this study, a systematic metabolic engineering strategy was applied to Escherichia coli...
Miao-Miao Hu, Shu-Fang Qin, Yi-Lu Zhang et al.· Journal of Agricultural and...· 0 citations
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