Determination Of The Lc50 Value Of Ethanol Extracts From Prasman Leaves (Eupatorium Triplinerve Vahl) On Artemia Salina Larvae Using The Bslt Method
TL;DR
Findings indicate the presence of bioactive compounds that may contribute to the plant’s biological activity and provide a scientific basis for further studies on its phytochemical constituents and potential pharmacological properties.
Abstract
Prasman (Eupatorium triplinerve Vahl) is a medicinal plant widely distributed in Eastern Indonesia and has long been traditionally used for various health-related purposes. The presence of secondary metabolites in this plant indicates potential biological activities that warrant further investigation, including toxicity assessment. This study aimed to determine the LC50 value of the ethanol extract of prasman leaves using the Brine Shrimp Lethality Test (BSLT) method. The extract was obtained through maceration using 96% ethanol as the solvent. Toxicity testing was conducted on Artemia salina larvae at concentrations of 10, 100, 500, 1000, 5000, and 10000 ppm. Each concentration was tested in duplicate with ten larvae in each treatment group. Larval mortality was observed after 24 hours of exposure, and the resulting data were used to determine the LC50 value of the extract. The results showed that the ethanol extract of prasman leaves had an LC50 value of 849,59 ppm. Based on this result, the ethanol extract of prasman leaves was classified as moderately toxic. These findings indicate the presence of bioactive compounds that may contribute to the plant’s biological activity and provide a scientific basis for further studies on its phytochemical constituents and potential pharmacological properties.