Sep 2026· Applied Biochemistry and Biotechnology· 0 citations· 36 references
Medicine
TL;DR
This one-tube, two-step fluorescent system presents a robust alternative for PRRSV diagnostics, combining specificity, sensitivity, and ease of use, well-suited for rapid field identification, molecular typing, and surveillance, thereby aiding in the implementation of targeted control measures.
The developed RPA-CRISPR/Cas12a assay overcomes the limitations of traditional diagnostic methods and holds great promise for on-site routine screening in poultry farms and epidemiological surveillance of MDV and REV.
Rui-Ying Han, Ning Xiao, Jia-Hua Wang et al.· Analytical Biochemistry· 0 citations
Avian leukosis virus subgroup J (ALV-J) is a retrovirus that poses a serious threat to the poultry industry. It is prone to genetic variation, leading to the emergence of multiple epidemic strains, which creates substantial challenges for clinical detection. Although the recombinase polymerase amplification (RPA)-CRI...
Rui-Ying Han, Ning Xiao, Zhi-Rui Wu et al.· ACS Infectious Diseases· 0 citations
The ERA-CRISPR/Cas12a method exhibits enhanced sensitivity for H5 and H7 subtypes detection, along with high specificity, rapidity, and minimal equipment requirements, and offers a promising screening tool for NIAV infections.
Zheng-Han Luo, Min-Zhi Xu, Le-Tian Zhang et al.· Frontiers in Cellular and In...· 0 citations
Cardamom mosaic virus (CdMV), associated with mosaic/katte disease in cardamom, is a highly destructive virus that leads to severe reductions in plant growth and yield. High levels of genetic variability approaching species-level divergence exist among CdMV isolates from different geographical regions. Development of a...
M. Greeshma, A. Bhat· Journal of Virological Metho...· 0 citations
This rapid and highly sensitive diagnostic assay was established for genotype VII NDV by integrating reverse transcription recombinase polymerase amplification with the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a system, in combination with a lateral flow dipstick (LFD) for visual signal r...
Ya-Ping Huang, Wei Li, Yu-Hui Song et al.· Frontiers in Veterinary Scie...· 0 citations
A rapid, field-deployable recombinase polymerase amplification (RPA)-CRISPR/Cas12a assay for the detection of African swine fever virus using both fluorescence- and lateral flow strip (LFS)-based readouts shows 100% analytical specificity against other porcine viral pathogens and strong agreement with real-time PCR.
P. Madhankumar, G. Sharma, K. G. Sai Balaji et al.· Archives of Virology· 0 citations
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