Aug 2026· ACS Applied Nano Materials· 0 citations· 36 references
TL;DR
This work presents an externally triggered multifunctional therapeutic platform that integrates photothermal bacterial eradication with antioxidant regulation, providing a promising strategy for chronic wound management.
Abstract
Chronic bacterial infections and oxidative stress severely impede wound healing, necessitating innovative strategies that simultaneously eradicate pathogens and modulate the hostile wound microenvironment. Herein, we develop a near-infrared (NIR)-responsive multifunctional nanoplatform (APDA-Pt) by integrating l-arginine-assisted polydopamine (APDA) with catalase-mimetic platinum nanoparticles (Pt NPs) to achieve synergistic photothermal antibacterial therapy and oxidative stress regulation. APDA serves as both a robust photothermal transducer and a versatile scaffold to anchor ultrasmall Pt NPs with high dispersion and enhanced catalytic utilization. Under NIR irradiation, APDA-Pt rapidly elevates local temperature, enabling efficient membrane disruption and broad-spectrum antibacterial efficacy (>99% inactivation of E. coli and S. aureus). Meanwhile, Pt NPs exhibit catalase-mimetic activity by catalyzing the decomposition of H2O2 into H2O and O2, whereas the APDA framework provides intrinsic antioxidant activity through its redox-active catechol groups, thereby synergistically alleviating oxidative stress. In an S. aureus-infected murine wound model, APDA-Pt combined with NIR irradiation markedly accelerates wound closure (≈90% by day 10), reduces bacterial burden by over 4 orders of magnitude, and promotes collagen deposition and tissue regeneration, without inducing systemic toxicity. This work presents an externally triggered multifunctional therapeutic platform that integrates photothermal bacterial eradication with antioxidant regulation, providing a promising strategy for chronic wound management.
This work developed a synergistic single-platform strategy for precise regulation of diabetic wound microenvironments, providing a promising therapeutic alternative for refractory diabetic wound treatment.
Chronic diabetic wounds represent a severe complication of diabetes mellitus and a prototypical form of chronic nonhealing wounds, characterized by biofilm-associated infection, persistent inflammation, and impaired angiogenesis. Herein, a multifunctional microneedle platform incorporating cationic chitosan-coated ruthenium dioxide nanozymes (RuO2@QCS NPs), termed RuO2@QCS-MN, is developed to accelerate diabetic wound healing through microenvironment reprogramming. This integrated system combines photothermal antibacterial activity, reactive oxygen species (ROS) scavenging, and in situ oxygen generation to coordinately regulate the pathological milieu of infected wounds. The microneedles effectively penetrate bacterial biofilms and deliver nanozymes to bacteria-enriched regions, enabling efficient yet mild photothermal antibacterial therapy. Meanwhile, RuO2@QCS NPs exhibit catalase-like activity, catalyzing endogenous hydrogen peroxide into oxygen, thereby enhancing nanozyme diffusion, alleviating oxidative stress, modulating inflammatory responses, and promoting macrophage polarization. Simultaneous oxygen generation may alleviate hypoxia and promote angiogenic responses. Both in vitro and diabetic in vivo models demonstrate efficient bacterial elimination, inflammation suppression, and enhanced re-epithelialization and neovascularization, ultimately accelerating wound repair. This work establishes a highly integrated nanozyme-enabled therapeutic paradigm for the localized treatment of infected diabetic wounds.
Xiang Li, Yuemiao Mao, Bo Ye et al.· ACS Applied Materials and In...· 0 citations
In diabetic wounds, diverse reactive oxygen species (ROS) intertwine to form a complex oxidative stress network, directly causing damage to cells and tissues. Most single-therapy materials fail to fully regulate this complex oxidative stress microenvironment, hindering wound healing. In this study, a nanozyme-hydrogel composite system was constructed. Through the complementarity of photothermal and enzyme-catalytic functions, cellular functions were enhanced while the oxidative stress microenvironment of diabetic wounds was alleviated, thereby collectively promoting wound healing. A cerium oxide (CeO2)-based nanozyme with abundant oxygen vacancies and high photothermal efficiency was fabricated via gadolinium (Gd) doping and platinum (Pt) nanocluster modification. Gd doping enhanced the catalase (CAT) -mimetic activity by increasing oxygen vacancies, while Pt nanoclusters enabled photothermal conversion. Through this functional complementarity, efficient wound regulation was achieved. To ensure stable retention, the nanozyme (GCP) was loaded into an in situ photo-cross-linkable hydrogel to form the GCP@PG dressing. In vitro and in vivo, GCP@PG scavenged intracellular ROS and promoted angiogenesis. In diabetic rats with full-thickness skin defects, the wound closure rate reached 97.95% on day 14. By integrating the functional complementarity of enzyme catalysis and photothermal effects, this composite system provides a strategy for diabetic wound repair.
Yang Yang, Dinping She, Shuya Zhang et al.· Chemistry of Materials· 0 citations
This work demonstrates a safe and effective strategy for combating MDR infections through the combined action of photothermal therapy and nanozyme catalysis, offering promising potential for clinical wound management.
Weiwei Zhang, Lixiang Fan, Xuanjun Zhang et al.· ACS Applied Materials and In...· 0 citations
A dual-functional nanoplatform constructed through the coordination-driven assembly of gallium ions (Ga3+) and polydopamine (PDA) for synergistic photothermal and metabolic antibiofilm therapy provides a promising therapeutic strategy for the management of biofilm-associated infections.
Xinyan Zheng, Jie Chen, Tongtong Yin et al.· Biomacromolecules· 0 citations
Methicillin-resistant Staphylococcus aureus (MRSA) infections represent a severe global clinical threat due to its multi-antibiotic resistance, abundant virulence factors, and complex pathogenic mechanisms. In the present study, hollow copper sulfide (CuS) nanoparticles (NPs) with a strong photothermal effect were employed as carriers for the loading of the NO-releasing bioactive molecule S-nitrosoglycine (GSNO). These NPs were also camouflaged with mouse red blood cell membranes (RBCMs) via extrusion to fabricate CuS-GSNO@RBCM NPs designed to effectively eliminate MRSA and its biofilms. Under 1064 nm near-infrared laser irradiation, CuS NPs had a mild photothermal effect, establishing an in situ catalytic platform that boosted reactive oxygen species (ROS) production. In the acidic infectious microenvironment, the release of copper ions induced GSNO to produce NO for reactive nitrogen species (RNS) generation. The ROS and RNS generated in situ by this system consumed endogenous MRSA glutathione, disrupting redox homeostasis, with the additional presence of copper ion-mediated copper death forming a multi-bactericidal network. The RBCMs also neutralized proteins secreted by MRSA to reduce lung cell injury. In vitro and in vivo experiments were employed to verify the MRSA-elimination efficacy of the proposed system, which offers an innovative nanotherapeutic strategy for the precise treatment of drug-resistant bacterial infections.
Xiangjun Chen, Sai Zhang, Yating Liu et al.· Small· 0 citations
