Complete genome sequence of Lacticaseibacillus rhamnosus B34-0-2 isolated from Panax ginseng in South Korea.

Complete genome sequence of Leuconostoc lactis B34-1-7-1 isolated from Panax ginseng in South Korea

Leuconostoc lactis is a heterofermentative lactic acid bacterium widely used as a starter culture in fermented foods. The L. lactis B34-1-7-1 strain, isolated from Panax ginseng sprouts cultivated under white LED light, has demonstrated promising probiotic potential, including antioxidant, anti-inflammatory, and anti-cancer activities, in previous studies. The genome of L. lactis B34-1-7-1 was sequenced and analyzed to evaluate its probiotic potential and safety for industrial applications, focusing on its genomic features and functional characteristics. The genome of L. lactis B34-1-7-1 was sequenced using a hybrid Illumina-PacBio approach. The complete genome is composed of a circular chromosome (1,648,351 bp) and a plasmid (12,481 bp), with an overall guanine-cytosine (GC) content of 43.6%. The genome encodes 1,661 coding sequences (CDSs), 68 transfer RNAs, and 12 ribosomal RNAs. Functional classification assigned 99.15% (1,647 genes) of the CDSs to Clusters of Orthologous Groups (COGs). Genome-based screening identified no known antibiotic resistance genes or virulence factors, supporting the safety of this strain. In addition, 23 genes associated with reductase and oxidase activities were identified, providing a genetic basis for its observed antioxidant potential. These results provide a foundational genomic resource supporting the application of L. lactis B34-1-7-1 in the functional food and probiotic industries.

Automated pangenomic profiling of Pediococcus acidilactici genomes via Snakemake: uncovering genomic plasticity and specialized antimicrobial potential

Genomic Insights Into Bacillus thuringiensis Strain JSd1 From Bangladesh: A Whole Genome Sequencing Approach

The findings suggest that B. thuringiensis JSd1 is a promising candidate for development as a biopesticide targeting insect pests in Bangladeshi agriculture, and lays the groundwork for further functional validation and field applications, contributing to sustainable pest management strategies in the region.

Bioprospection of Mammaliicoccus sciuri Strain TRQ48 as a Plant-Growth-Promoting Bacteria Associated with Wheat (Triticum turgidum L. subsp. durum) in the Yaqui Valley, Mexico

Bioprospecting of plant growth-promoting bacteria enables the identification of beneficial microbial resources with significant potential for sustainable agricultural applications. In this context, strain TRQ48 was isolated from a commercial field of wheat (Triticum turgidum L. subsp. durum) located in the Yaqui Valley, Mexico, with the aim of exploring its plant growth-promoting potential. The draft genome sequence presented a genomic size of 2,777,016 bp, 32.5% G + C content, 665,763 bp N50, 2 L50, and 19 contigs. Taxonomic affiliation demonstrated that strain TRQ48 belonged to Mammaliicoccus sciuri. Genome annotation identified 2756 coding DNA sequences (CDS) distributed into 259 subsystems, highlighting CDS associated with iron acquisition and metabolism, stress response, and virulence, disease, and defense, among others. Metabolic assays reflect the strain’s capacity to produce siderophores and auxins, relating these positive traits to significantly (p ≤ 0.05) promote growth of wheat shoot length (7.09%) and root and shoot dry weight (75% and 18.43%) compared to uninoculated wheat plants. Biosafety testing indicated that the strain is susceptible to commonly used antibiotics and lacks clinically relevant resistance profiles, matching genomic analysis, which did not reveal any critical virulence factors. Thus, although the presented results show that M. sciuri TRQ48 is a promising beneficial biosafe strain, further studies are still needed to evaluate its performance under agro-ecosystems at commercial levels.

Genomic characterization of Bacillus velezensis BP5 and BP103: insights into biocontrol potential against bacterial leaf spot on pepper

Findings establish B. velezensis strains BP5 and BP103 as highly promising biocontrol agents, combining high genetic stability with diverse secondary metabolite profiles, suitable for development into sustainable microbial bio-bactericides.

Comparative genomics of Bifidobacterium crudilactis NASR_001 – unveiling the tapestry of a putative probiotic

The selective isolation and whole genome sequencing (WGS) of Bifidobacterium crudilactis from a pure culture of dairy origin from India offers valuable insights for further exploration of its promising probiotic potential and functional characteristics.