Sep 2026· Chemical Research in Chinese Universities· Vol 42, pp. 1519 - 1526· 0 citations· 42 references
TL;DR
A functionalized carbon nanopipette platform for fluorescence-based analysis of relative changes in intracellular protein kinase A (PKA) activity at the single-cell level is developed and provides a modular platform that may be extended to other phosphorylation-related signaling events by changing the peptide substrate.
Monitoring protein kinases in their native environment is essential for understanding cellular signaling. This study introduces triangulenium-based peptide biosensors for detection of the activity of two key protein kinases involved in T cell immune response: Akt1 and Src. The substrate biosensors incorporate a long-li...
Carmen Salto-Giron, Angélica Martín-Priego, Francisco Fueyo-González et al.· Small· 0 citations
Both in vitro experiments and in situ lysosomal K+ imaging along with external pharmacological stimuli demonstrated the excellent performance of the proposed K+ nanosensor, which expanded the application of DNA-based probes in subcellular imaging field.
Guangming Li, Ying An, Jia-Hui Sun et al.· Biosensors & bioelectronics· 0 citations
A cell-permeable fluorogenic analogue of the phosphodiesterase 4 (PDE4) inhibitor rolipram (MAN193), generated by conjugation to fluorescein diacetate is developed, establishing fluorogenic drug conjugation combined with quantitative spectroscopy imaging as a generalisable strategy to map intracellular drug–target enga...
Immunofluorescence (IF) staining is widely used to visualize biomolecules in cells and tissues. Conventional IF typically relies on fluorophore-conjugated antibodies, whose preparation requires chemical modification procedures that can compromise antibody performance and incur product loss during subsequent purificatio...
Xin-Xin Xu, Zheng-Mao Kong, Zhi-Peng Su et al.· In Analysis· 0 citations
Understanding whether internalized nanoparticles remain confined within intracellular vesicles or are released into the cytosol is essential for evaluating their biological activity and potential use as delivery systems. However, this distinction is often difficult to establish by using fluorescence imaging alone, part...
Karina Kwapiszewska, G. Bubak, Krzysztof Bielec et al.· Journal of Physical Chemistr...· 0 citations
A novel reconstitution platform is developed that exploits the membrane fusion capability of extracellular vesicles to incorporate membrane proteins into planar lipid bilayers with high efficiency and preferential orientation and provides a versatile foundation for membrane protein analysis, drug discovery, and the eng...
