Skip to content
Log in
Sponsored Artificial Intelligence: A Modern Approach, Global Edition Artificial Intelligence: A Modern Approach, Global Edition Amazon

Similar papers

Jul 2026

High-cell-density fermentation of Komagataella phaffii enables the overproduction of a novel, broad-substrate endoglucanase, WcEG394 from Fuling (Wolfiporia cocos), for lignocellulose biorefining.

Recombinant WcEG394 efficiently hydrolyzed carboxymethyl cellulose, microcrystalline cellulose, and filter paper, and demonstrated broad substrate specificity toward xylans, pectin, chitin, agarose and agricultural residues such as corn cob and sugarcane bagasse.

Hongbo Li, Wenling Yan, Lingzhi Tang et al. · 0 citations
Open access Jul 2026

Tri-valorization of methanol in a single bioreactor: co-production of enzyme, chemical, and single-cell protein using engineered Pichia pastoris (Komagataella phaffii)

The economic viability of methanol-based biomanufacturing, particularly with green methanol as feedstock, is often limited by the low value of single-product processes. Here, we developed an integrated co-production strategy in the methylotrophic yeast Pichia pastoris (Komagataella phaffii) for the simultaneous conversion of methanol into three products: the sweetener erythritol, the industrial biocatalyst β-mannanase, and single-cell protein (SCP) biomass. This new strategy explores the inherent spatial and functional separation between the ER-Golgi secretory pathway for enzyme production and the cytosolic pathway for chemical synthesis, thereby reducing interference between the two pathways. The engineered co-production strain achieved β-mannanase and erythritol titers comparable to those of the corresponding β-mannanase- and erythritol-producing reference strains in both shake-flask and fed-batch fermentor cultures. Furthermore, transcriptomic analysis revealed distinct regulatory responses related to enzyme production and erythritol synthesis, supporting the limited cross-pathway interference between the two pathways. In addition, we showed that ultrafiltration enabled efficient downstream separation of the small-molecule erythritol from the secreted β-mannanase, with recovery efficiencies exceeding 89%. These results demonstrate a feasible strategy for methanol valorization into multiple value-added products and expand the potential of methylotrophic yeasts for integrated biomanufacturing.

Jiayu Fang, Shuxian Wang, Guoxia Liu et al. · 0 citations
Open access Jul 2026

Heterologous Expression of a Neurospora crassa Catalase Reprograms the Cellulase System and Enhances β-Glucosidase Production in Trichoderma reesei

Efficient saccharification of lignocellulose, the most abundant renewable carbon reservoir resource, is of great industrial importance. Trichoderma reesei is a premier cellulase producer, but its fermentation efficiency is often constrained by dual challenges: dissolved oxygen limitation and intrinsic oxidative stress. To address this, we engineered T. reesei to heterologously express a robust catalase gene (cat-3) from Neurospora crassa. The recombinant strain Tr-cNcat3 exhibited a 7.4-fold increase in extracellular catalase activity. Tr-cNcat3 showed an increase in total extracellular protein, resulting in markedly enhanced filter paper activity (FPA) and β-glucosidase activity compared to the control. Strikingly, this intervention specifically triggered a significantly higher expression of β-glucosidase, a known bottleneck in T. reesei’s cellulase system, particularly on bagasse and straw as the carbon source. Moreover, the ability of the supernatant to degrade cellulose substrates was improved. Our results reveal that overexpression of cat-3 in T. reesei could modify the cellulase cocktail by triggering a higher level of β-glucosidase. This study provides a novel and effective genetic engineering strategy to unlock the full industrial potential of T. reesei for cost-effective lignocellulosic biorefining.

Haowen Sun, Chang-Bin Tang, Yifan Chen et al. · 0 citations
Open access Aug 2026

Multistrategy metabolic engineering of Talaromyces pinophilus for α-amylase production from lignocellulosic biomass

Metabolic engineering of Talaromyces pinophilus through promoter optimization, multicopy integration, and protease deletion enables efficient α-amylase production from lignocellulosic biomass, achieving 26 712 U/mL in bioreactor fermentation.

Jing Zeng, Jianjun Guo, Shuaiwen Zhang et al. · 0 citations
Jul 2026

High-density cultivation of Lacticaseibacillus paracasei GY-1 in a novel medium produces a structurally distinct immunostimulatory exopolysaccharide.

Lactic acid bacteria are important probiotics and producers of valuable released exopolysaccharides (r-EPS). However, achieving high cell density cultivation (HCDC) and high r-EPS yields in a simple batch culture remains challenging, partly due to the lack of an effective medium. To address this gap, we developed a novel multifunctional Spirulina platensis (SP) broth, designated MSP, for cultivating Lacticaseibacillus paracasei GY-1 by replacing all nitrogen sources (peptone, beef extract, and yeast extract) in De Man, Rogosa, and Sharpe (MRS) broth with 15.48 g/L SP hydrolysates, supplemented with 0.5 g/L corn steep liquor and 1 g/L trehalose. MSP achieved an unprecedented HCDC of 1.72 × 1011 viable cells/mL (70-fold higher than MRS) in 20 h, thereby increasing total r-EPS yields by 4.16 times. Purification identified two MSP-specific fractions: r-EPS1 and r-EPS2. In vitro assays demonstrated that r-EPS2 significantly promoted RAW264.7 cell proliferation and cytokine secretion (IL-1β, TNF-α, IL-6, and IL-10) compared to r-EPS1 and lipopolysaccharide. Structural characterization identified r-EPS2 as a novel polysaccharide (7.66 × 105 Da) composed of 98.90% glucose, with a backbone of (1 → 4)-α-D-Glcp and branches of (1 → 4,6)-α-D-Glcp and (1 → 3,4)-α-D-Glcp. Collectively, MSP is the first versatile medium that enables HCDC, enhances r-EPS yield, and produces structurally distinct r-EPS with potent immunostimulatory activity in a single batch culture, highlighting its potential for probiotic and r-EPS applications.

Yizhi Zou, Xiaona Xu, Sihan Zhang et al. · 0 citations
Jul 2026

Characterization of a Novel GH3 β-Glucosidase with Alkali-Tolerant and Halophilic Properties from Pontixanthobacter luteolus SW-109.

Local enrichment of acidic residues on the PlGH3 surface could generate a negative electrostatic potential, which enables adaptation to high-salt and alkaline environments, thereby sustaining the enzyme's catalytic activity under such extreme conditions.

Kaijuan Wu, Ke Guo, Zheng Yu et al. · 0 citations