Leucobacter shiyiweii sp. nov. and Leucobacter fengyongliangi sp. nov., isolated from coal samples in Shanxi Province of China.

Polyphasic and genomic characterisation of Streptomyces zimensis sp. nov., a halotolerant Actinomycetota from the Saline Lake Zima in Morocco

Members of the phylum Actinomycetota are widely distributed across diverse environments and are well known for their metabolic versatility and capacity to produce bioactive compounds. In this study, strain ZE1316R2Aᵀ was isolated from the saline water collected from Lake Zima (Morocco) and subjected to comprehensive polyphasic taxonomic characterisation. Phylogenetic analysis based on the 16 S rRNA gene placed strain ZE1316R2Aᵀ within the genus Streptomyces , showing highest sequence similarity with S. albidoflavus DSM 40,455 T (99.71%). However, genome-based indices, including average nucleotide identity (ANIb = 94.84%, ANIm = 96.09%) and digital DNA-DNA hybridization (dDDH = 64.9%), supported its distinction as a separate species. The draft genome (7.41 Mb; G + C = 73.26 mol%) comprises 6,464 coding sequences and reveals the presence of strain-specific genomic regions and biosynthetic gene clusters. Comparative analyses highlighted both a conserved core genome and a substantial accessory genome component, reflecting genomic differentiation relative to closely related taxa. Phenotypic and chemotaxonomic characteristics were consistent with assignment to the genus Streptomyces , while supporting its differentiation at the species level. Based on the combined genomic, phenotypic, and chemotaxonomic evidence, strain ZE1316R2Aᵀ represents a novel species of the genus Streptomyces , for which the name Streptomyces zimensis sp. nov., is proposed. This study expands current knowledge of Streptomyces diversity associated with saline environments and highlights the genomic diversity present within closely related taxa. The type strain is ZE1316R2Aᵀ (= CCMM B1331 T  = DSM 120541 T ).

Microbacterium altimontanum sp. nov., Microbacterium longlingense sp. nov., and Microbacterium cucumeris sp. nov.: Three New Species of Microbacterium Isolated from Soil in Yunnan Province, China

Three actinobacterial strains, AW10-10T, M8-1T and HGHZ1-2T, were isolated from soil in Yunnan Province, China. Analyses of 16S rRNA gene sequences and shotgun-sequenced draft genomes placed them within the genus Microbacterium. OrthoANI, FastANI and dDDH values between the three strains and their closest relatives were 75.7–79.6%, 79.9–81.9% and 19.7–22.5%, respectively, all well below the accepted species-level thresholds. Cells of all three strains were Gram-stain-positive, aerobic and rod-shaped. The major fatty acids were anteiso-C17:0, anteiso-C15:0 and iso-C16:0 in AW10-10T and HGHZ1-2T, and anteiso-C15:0, anteiso-C17:0 and iso-C16:0 in M8-1T. AW10-10T and M8-1T contained ribose, galactose, mannose, glucose and minor rhamnose, whereas HGHZ1-2T lacked rhamnose. The predominant menaquinones were MK-12 and MK-13 in AW10-10T, MK-13 and MK-12 in HGHZ1-2T, and MK-10, MK-11 and MK-9 in M8-1T. Polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid in AW10-10T and HGHZ1-2T, but two unidentified glycolipids in M8-1T. DNA G + C contents were 70.0, 69.7 and 69.5 mol%, respectively. The strains represent three novel species, Microbacterium altimontanum sp. nov., Microbacterium longlingense sp. nov. and Microbacterium cucumeris sp. nov., with type strains AW10-10T (=GDMCC 16749T = KCTC 59693T), M8-1T (=GDMCC 16752T = KCTC 59696T) and HGHZ1-2T (=GDMCC 16751T = KCTC 59694T), respectively.

Altererythrobacter chaiwopuensis sp. nov., a novel alphaproteobacterial strain isolated from sediment of the Chaiwopu Lake

Abstract Two alphaproteobacterial strains, designated EGI L300112T and EGI L300120, were isolated from sediment collected from Chaiwopu Lake in Xinjiang, China. The taxonomic position of the two strains was determined using polyphasic taxonomic analysis and phylogenomic analysis. Based on 16S rRNA gene sequence similarities, strain EGI L300112T and EGI L300120 were both closely related to Pelagerythrobacter rhizovicinus AY-3RT and shared the highest sequence identities of 96.2% and 96.3%, respectively. However, phylogenetic analysis based on the 16S rRNA gene and genomes clearly demonstrated that the two strains formed a distinct clade with validly published species of the genus Altererythrobacter. Furthermore, the two strains exhibited distinct phenotypic, physiological and genotypic characteristics that differentiate them from other related type species of the genus Altererythrobacter and related species in the family Erythrobacteraceae. Cells of the two strains were aerobic, Gram-stain negative, non-motile and rod-shaped. Optimal growth conditions for EGI L300112T and EGI L300120 occurred on marine agar 2216 at pH 7 at 30 °C. The major respiratory quinone was Q-10. The detected polar lipids of two strains included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and unidentified phospholipids. The major fatty acids (>10%) were identified as summed feature 3 (C16 : 1ω7 c/C16 : 1ω6 c) and summed feature 8 (C18 : 1ω7 c). The G+C content of strain EGI L300112T and EGI L300120 was 61.2% and 61.4%, respectively. Based on differential phenotypic and genotypic characteristics of the two strains and related species in the genus Altererythrobacter, the two strains should be classified as representing a new species of this genus, for which the name Altererythrobacter chaiwopuensis sp. nov. is proposed. The type strain is EGI L300112T (=MCCC 1K09432T=KCTC 8712T).

Description and genomic characterization of Aquipuribacter aurantiacus sp. nov., isolated from saline lake sediment.

Strains MA13-6T and MA13-13, two Gram-stain-positive, aerobic, short rod-shaped actinobacteria, were isolated from a saline lake in Ngari Prefecture, Xizang Autonomous Region, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belonged to the genus Aquipuribacter, with the closest relationship to Aquipuribacter hungaricus IV-75T (98.47% sequence similarity) and Aquipuribacter nitratireducens AMV4T (97.36% sequence similarity). Phylogenetic analysis based on genomes further confirmed their classification as a distinct cluster within the genus Aquipuribacter. The average nucleotide identity and digtal DNA-DNA hybridization values between these two strains and their closest relative Aquipuribacter hungaricus IV-75T, were 82.44-82.49% and 23.00%, respectively, clearly indicating that strains MA13-6T and MA13-13 represent a novel species. The 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA-DNA hybridization values between these two strains were 99.79%, 99.97% and 99.40%, respectively, unequivocally confirming their classification within the same species. However, DNA fingerprinting analysis distinguished them as non-clonal variants. The polar lipids comprised phosphatidylglycerol, two unidentified phospholipids, two unidentified glycolipids, and two unidentified lipids. The predominant respiratory quinone was MK-10 (H4). The major fatty acids were anteiso-C15:0, C18:1ω9c, isoC16:0 and anteiso-C17:0. The cell wall diagnostic diamino acid was meso-diaminopimelic acid. Based on phylogenetic analyses combined with phenotypic and chemotaxonomic characterization, strains MA13-6T and MA13-13 represent a novel species of the genus Aquipuribacter, for which the name Aquipuribacter aurantiacus sp. nov. is proposed. The type strain is MA13-6T (=MCCC 1K10045T = KCTC 59572T).

Pseudoalteromonas nanhaiensis sp. nov., a Novel Bacterium Isolated from South China Sea

Whole genome sequencing and molecular characterization of two Bacillus licheniformis strains isolated from hot springs in yellowstone ational park

Two B. licheniformis strains isolated from extreme environments are isolated from the Five Sisters hot spring in Yellowstone National Park and whole-genome sequencing reveals an open pangenome of 10,374 genes, providing a genomic foundation for future exploration of novel bioactive compounds with potential applications in drug discovery, agriculture, and biotechnology.