Evaluation of eef1a1l1 expression stability and promoter-driven reporter activity during zebrafish development

Abstract

Housekeeping genes are widely used as experimental references for gene expression, yet many exhibit substantial variation within and between cell types. Using Zebrafish Meta Atlas Project (ZMAP), we found eef1a1l1 to be ubiquitously and highly expressed across zebrafish embryonic cell types, developmental stages, and studies. Transgenic reporters driven by 2.2-kb or 2.6-kb eef1a1l1 -derived regulatory sequences showed broad embryonic expression, and single-cell profiling revealed that reporter abundance covaried with that of the endogenous eef1a1l1 transcript. These findings further support eef1a1l1 as a reference for embryonic gene expression and the use of its regulatory sequence as a practical, multipurpose transgenic driver.