A dual-signal lateral flow biosensing platform for visual detection of miRNA-21 in serum.

Polydopamine nanoparticle-based colorimetric–photothermal dual-mode lateral flow immunoassay for thromboembolism diagnosis

A dual-mode colorimetric–photothermal LFIA platform was successfully constructed for the detection of thrombosis-related biomarkers, including thrombomodulin and α2-plasmin inhibitor–plasmin complex, in clinical serum samples, enhancing analytical sensitivity and reliability.

Dual-Mode Lateral Flow Strip with Förster Resonance Energy Transfer for Rapid and Accurate Thrombin Detection

A dual-aptamer-based, dual-mode FRET lateral flow strip is developed for the rapid and sensitive detection of thrombin in serum samples, achieving a fluorescence-based detection limit of 0.135 nM-twice as sensitive as the colorimetric method.

A portable smartphone-based fluorescence biosensing strategy for exosome detection

Exosomes play a crucial role in intercellular communication and have emerged as promising biomarkers for disease diagnosis and prognosis, however, their nanoscale size and low abundance in biological fluids pose substantial challenges for rapid and quantitative detection. Conventional detection techniques rely on bulky instrumentation and complex workflows that hinder point-of-care implementation. Here, we report a portable smartphone-based fluorescence sensing platform specifically designed to address the limitations of existing exosome assays in portability, quantitative robustness, and operational simplicity. Exosome capture was achieved through a CD63-specific aptamer, followed by molecular recognition using a FAM-labeled, tumor-specific aptamer. A miniaturized optical readout device integrating an excitation unit, optical filters, and an imaging module enables fluorescence acquisition and quantitative analysis without large laboratory equipment. The system completes exosome detection within 45 min and achieves a limit of detection of 4.58 × 106 particles/ml. Clinical sample analysis preliminarily demonstrates the feasibility of serum exosome detection using this portable platform, suggesting its potential utility for rapid, on-site biomedical screening.

Dual-readout lateral flow immunoassay based on upconversion nanoparticle-gold nanoparticle luminescence resonance energy transfer for rapid determination of 17β-estradiol

A lateral flow assay strip based on AND logic gate and DNA self-assembly for dual miRNA detection in breast cancer diagnosis

Multiplexed Detection of Breast Cancer-Specific miRNA and Protein Biomarkers on Exosomes Using a Microfluidic Platform.

Exosomes, rich in diverse bioactive molecules, have emerged as a promising "liquid biopsy" approach for early cancer screening. However, relying solely on a single biomarker type can easily lead to missed diagnoses or misjudgments. Therefore, there is an urgent need to develop multiple biomarkers detection strategies to facilitate precise diagnosis. Herein, a microfluidic chip integrating two signals (ALP-driven chemiluminescence and nanobeacons-based fluorescence) into a single device was developed using breast cancer exosomes as a model. A simple sample injection enabled capture and quantitative detection of exosomal surface proteins (CD63, EpCAM, HER2) and internal miRNAs (miRNA-21, miRNA-375). After capture, protein levels were determined by driving magnetic beads and capturing images with a smartphone. Prefabricated nanobeacons were then directly introduced into the chip, enabling miRNA signal detection without RNA extraction. This five-marker biomarker panel achieved a diagnostic accuracy of 99.6% in breast cancer identification, representing an improvement of at least 4.6% over single-marker approaches and an 8.6% increase over any individual biomarker. This multitarget exosome analysis platform may provide a highly sensitive, low-cost, and easily scalable novel tool for breast cancer early diagnosis and treatment efficacy assessment.