Aug 2026· International Journal of Molecular Sciences· Vol 27, pp. 7604· 0 citations· 51 references
TL;DR
Findings substantiate a positive regulatory role of Mn-Tret1 in governing growth performance in M. nipponense and contributes to a deeper mechanistic understanding of growth regulation in this freshwater prawn species.
Abstract
Macrobrachium nipponense is a commercially significant freshwater prawn species in China, with larger individuals commanding higher market prices than smaller ones. Previous transcriptomic analyses have shown that a facilitated trehalose transporter gene Tret1-like (Tret1) exhibits significantly elevated expression levels in fast-growing individuals compared to their slow-growing counterparts, suggesting a potential involvement of this gene in modulating growth performance. In the present study, we explored the functional roles of Mn-Tret1 in growth regulation using reverse transcription quantitative real-time PCR (RT-qPCR) and RNA interference (RNAi) techniques. The full-length cDNA sequence of Mn-Tret1 spans 1605 bp and encodes a 535 amino acid (aa) protein. Phylogenetic analysis indicated that the deduced amino acid sequence of Mn-Tret1 shares the closest evolutionary relationship with that of Macrobrachium rosenbergii. Tissue distribution analysis via RT-qPCR revealed that Mn-Tret1 is ubiquitously expressed across all examined tissues, implying its potential involvement in multiple physiological processes. Notably, the highest transcript abundance was detected in muscle tissue, further suggesting its putative role in governing growth performance in M. nipponense. Subsequent RT-qPCR analysis validated the knockdown efficacy of the synthesized dsTret1 on Mn-Tret1 expression. Compared with the dsGFP-injected control group, the dsTret1-injected group exhibited a statistically significant reduction in body mass gain percentage, with the divergence becoming apparent from day 6 in females and day 12 in males (p < 0.05). Moreover, histopathological examination revealed abdominal muscle atrophy in individuals subjected to dsTret1 treatment. Collectively, these findings substantiate a positive regulatory role of Mn-Tret1 in governing growth performance. This study thereby contributes to a deeper mechanistic understanding of growth regulation in M. nipponense.
Sucrose non-fermenting-1-related protein kinase (SnRK) is a plant serine/threonine kinase that mediates stress signaling, yet its function in the mangrove Avicennia marina remains unexplored. In this study, we identified 46 SnRK genes in A. marina, classifying them into three subfamilies with high phylogenetic conservation. Family expansion occurred mainly through 20 segmental duplication events under purifying selection (Ka/Ks < 1). Promoter regions were enriched in stress- and hormone-responsive elements, and expression profiling showed distinct tissue-specific and stress-responsive patterns. Our study specifically focused on the functional characterization of AmSnRK2.7. AmSnRK2.7-overexpressed Arabidopsis thaliana increases salt tolerance by altering the expression of ion transport genes and mediating Na+ efflux from the roots. Further investigation revealed that AmSnRK2.7 interacts with and phosphorylates AmENO2 (enolase, a key enzyme in the glycolysis pathway). Meanwhile, measurements of ATP content in wild type, AmSnRK2.7-overexpressed Arabidopsis lines and the atsnrk2.6 (homologous gene of AmSnRK2.7 in A. thaliana) mutant confirm that the AmSnRK2.7-AmENO2 module can affect the energy-driven Na+ efflux. Our findings provide key insights into the role of SnRK gene family in mangrove adaptation to saline intertidal habitat, and suggest AmSnRK2.7-AmENO2 module plays a role in salt tolerance.
Jin-Yu Liu, Yudan Xiang, Lingyu Song et al.· Plant, Cell and Environment· 0 citations
Toll-like receptors (TLRs) are important pattern recognition receptors involved in crustacean innate immunity. In this study, the full-length cDNA of
HtTLR2
was cloned from the mudflat crab
Helice tientsinensis
using rapid amplification of cDNA ends (RACE). The
HtTLR2
cDNA was 3854 bp in length and contained a 3036-bp open reading frame (ORF) encoding a 1011-amino-acid protein. Bioinformatics analysis showed that the HtTLR2 protein possessed conserved structural features typical of TLR family members. Phylogenetic analysis indicated that HtTLR2 was closely related to Toll2 from
Eriocheir sinensis
. Tissue distribution analysis revealed high
HtTLR2
expression in the gills and hepatopancreas and its expression was significantly upregulated in both tissues following infection with
Vibrio parahaemolyticus
(Fujino et al., 1951) Sakazaki et al., 1963. This study provides new insights into the
TLR
gene family of
H. tientsinensis
and lays a foundation for further investigation of the immune mechanisms of this species.
Lulu Chen, Youkun Fang, Yang Ge et al.· Crustaceana· 0 citations
Pectin acetylesterase (PAE) regulates pectin acetylation, which affects plant growth, development, and stress tolerance. While their functions are well-defined in models like Arabidopsis, we still know surprisingly little about how they operate in tomatoes (Solanum lycopersicum). We identified 17 SlPAE genes using tomato genome-wide analysis. These genes were classified phylogenetically into three conserved subfamilies, with branch members sharing domain architectures, motifs, and genomic structure. Promoter cis-element analysis identified multiple motifs related to hormone signaling, light response, and stress response. Spatiotemporal expression patterns obtained via qRT-PCR revealed the functional roles of SlPAE genes. Notably, silencing SlPAE16 effectively retarded pedicel abscission, a process mediated by the inhibition of TAPG1/2/4 expression. These findings clarify the functional diversity within the PAE gene family, providing a much-needed framework for future research into their specific biological roles in tomatoes.
Ruizhen Li, Lin Shen, Jianzhong Tie et al.· BMC Plant Biology· 0 citations
Findings suggest that BmRNF181 acts as a negative regulator of the silkworm immune response and dramatically increased the expression of antimicrobial peptide genes.
Jiang Liu, Ying Peng, Ming Yu et al.· Journal of Invertebrate Path...· 0 citations
S-domain receptor-like kinases (S-RLKs) represent a typical RLK subfamily, which plays key roles in various biological processes in plants. However, the genome-wide evolutionary and functional differentiation of this family remains unclear in rice. In the present study, a comprehensive computational analysis was employed and identified 109 S-RLKs in 9311 genome and 103 S-RLKs in Nipponbare (Nip) genome. The S-RLKs were unevenly distributed across 12 chromosomes. Bioinformatics analysis indicate large-scale gene duplication and family expansion may be the main driving forces for the expansion of S-RLK family members. Although the S-RLKs are highly conserved between the two subspecies, it remains unknown whether these members have undergone functional differentiation during long-term evolution. Given the differences of roots and nitrogen (N) utilization in 9311 and Nip, we focused on the S-RLKs which exhibit different expressions in roots. OsNRS1 (Nitrogen-responsive Root S-domain kinase 1) was selected due to its expression in the roots of 9311 notably more than that in Nip. Phenotypic analysis showed that OsNRS1 CRISPR/Cas9 mutants in 9311 significantly inhibited the length of primary root and total root, and N accumulation, whereas OsNRS1 CRISPR/Cas9 mutants in Nip showed significant functional divergence. In-depth research revealed that these functional divergence of OsNRS1 may be caused by the sequence variation of an auxin response element AuxRR in its promoter. Collectively, this study not only provides novel insights into the evolution of the S-RLK gene family, but also identifies OsNRS1 as a potential key target for the genetic improvement of root and N utilization in rice.
Cong Chen, Jiajia Jin, Shujie Shi et al.· Plant physiology and biochem...· 0 citations
An extensive genome-wide study of the DUF668 gene family in potato demonstrated that StDUF668s play a role in crucial biological processes, involving abiotic and biotic stress responses, and provided a valuable resource for future research aimed at improving potato stress tolerance and growth.
Aiana Gill, Tanvi Mongia, Garima Kakkar et al.· Journal of Applied Genetics· 0 citations
