Agromyces capsici sp. nov., Agromyces cucurbitae sp. nov., and Agromyces solani sp. nov.: Three New Species Isolated from Rhizosphere Soils in Yunnan Province, China
Aug 2026· Microorganisms· Vol 14, pp. 1776· 0 citations· 64 references
Medicine
TL;DR
Three Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strains, designated LJC-15T, NGA-4T and QZ2-6-4T, were isolated from the rhizosphere soils of Capsicum, Cucurbita and Solanum melongena, respectively, in Yunnan Province, China and were proposed to represent three novel species of the genus Agromyces.
Abstract
Three Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strains, designated LJC-15T, NGA-4T and QZ2-6-4T, were isolated from the rhizosphere soils of Capsicum, Cucurbita and Solanum melongena, respectively, in Yunnan Province, China. Phylogenetic and phylogenomic analyses placed the three strains within the genus Agromyces but distinguished them from recognized species. All ANI and dDDH values between the novel strains and phylogenetically related type strains were well below the generally accepted species delineation thresholds. The three strains could also be differentiated from selected reference type strains by phenotypic characteristics. Chemotaxonomically, they contained MK-12 as the predominant menaquinone and anteiso-C15:0, anteiso-C17:0 and iso-C16:0 as the major fatty acids, while their whole-cell sugar and polar lipid profiles were consistent with those characteristic of the genus Agromyces. On the basis of the combined phylogenetic, genomic, phenotypic and chemotaxonomic evidence, strains LJC-15T, NGA-4T and QZ2-6-4T are proposed to represent three novel species of the genus Agromyces, for which the names Agromyces capsici sp. nov., Agromyces cucurbitae sp. nov. and Agromyces solani sp. nov. are proposed, respectively. The type strains are LJC-15T (=GDMCC 1.6754T = KCTC 59690T), NGA-4T (=GDMCC 1.6755T = KCTC 59691T) and QZ2-6-4T (=GDMCC 1.6756T = KCTC 59692T), respectively.
Three actinobacterial strains, AW10-10T, M8-1T and HGHZ1-2T, were isolated from soil in Yunnan Province, China. Analyses of 16S rRNA gene sequences and shotgun-sequenced draft genomes placed them within the genus Microbacterium. OrthoANI, FastANI and dDDH values between the three strains and their closest relatives were 75.7–79.6%, 79.9–81.9% and 19.7–22.5%, respectively, all well below the accepted species-level thresholds. Cells of all three strains were Gram-stain-positive, aerobic and rod-shaped. The major fatty acids were anteiso-C17:0, anteiso-C15:0 and iso-C16:0 in AW10-10T and HGHZ1-2T, and anteiso-C15:0, anteiso-C17:0 and iso-C16:0 in M8-1T. AW10-10T and M8-1T contained ribose, galactose, mannose, glucose and minor rhamnose, whereas HGHZ1-2T lacked rhamnose. The predominant menaquinones were MK-12 and MK-13 in AW10-10T, MK-13 and MK-12 in HGHZ1-2T, and MK-10, MK-11 and MK-9 in M8-1T. Polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid in AW10-10T and HGHZ1-2T, but two unidentified glycolipids in M8-1T. DNA G + C contents were 70.0, 69.7 and 69.5 mol%, respectively. The strains represent three novel species, Microbacterium altimontanum sp. nov., Microbacterium longlingense sp. nov. and Microbacterium cucumeris sp. nov., with type strains AW10-10T (=GDMCC 16749T = KCTC 59693T), M8-1T (=GDMCC 16752T = KCTC 59696T) and HGHZ1-2T (=GDMCC 16751T = KCTC 59694T), respectively.
Wenqi Lai, Xiankun Zhang, Z. Duan et al.· Microorganisms· 0 citations
It is confirmed that strain C159T is a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt and represents a novel species in the genus Pseudoneobacillus sp.
Shengfeng Pan, Zengwei Feng, Meng Chen et al.· International Journal of Sys...· 0 citations
Bacterial strains were isolated from different locations and screened for plant growth promoting (PGP) features. Comparative analyses of the 16S gene sequences of 5 of the strains indicated taxonomic relatedness within the genus Paenibacillus. A polyphasic taxonomic approach was employed to study the strains in detail to clarify their phylogenetic position. Genome-based analyses, including digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI), revealed values consistently below the accepted species delineation thresholds when compared with closest relatives of each strain. Additionally, the strains showed clear differences in their physiological and biochemical profiles to the type strains of the closest related species. A notably diverse set of genes potentially involved in plant growth promotion was detected in all strains. With respect to the analyses reported here, the following new names are proposed: Paenibacillus corni sp. nov., with AK-167T as the type strain (= LMG 34403T = DSM 121689T); Paenibacillus vaccinii sp. nov., with AK-264T as the type strain (= LMG 34404T = DSM 121714T); Paenibacillus ericacearum sp. nov., with AK-265T as the type strain (= CCM 6913T = LMG 34405T = DSM 121715T); Paenibacillus polytrichii sp. nov., with AK-286T as the type strain (= CCM 9614T = LMG 34406T = DSM 121716T); and Paenibacillus artemisiae sp. nov., with DT-106T as the type strain (= LMG 34409T = CCM 9610T = DSM 121690T).
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Genome analysis identified genetic features potentially associated with stress adaptation and iron/tryptophan metabolism, providing additional ecological context for this plant-associated isolate.
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Strain SX92 T is proposed as the type strain of a novel species, Streptomyces songxianensis, and is established as a promising multifunctional biocontrol agent for sustainable tobacco production.
Mengyu Zhang, Lei He, Chengjun Li et al.· Frontiers in Microbiology· 0 citations
Abstract Two alphaproteobacterial strains, designated EGI L300112T and EGI L300120, were isolated from sediment collected from Chaiwopu Lake in Xinjiang, China. The taxonomic position of the two strains was determined using polyphasic taxonomic analysis and phylogenomic analysis. Based on 16S rRNA gene sequence similarities, strain EGI L300112T and EGI L300120 were both closely related to Pelagerythrobacter rhizovicinus AY-3RT and shared the highest sequence identities of 96.2% and 96.3%, respectively. However, phylogenetic analysis based on the 16S rRNA gene and genomes clearly demonstrated that the two strains formed a distinct clade with validly published species of the genus Altererythrobacter. Furthermore, the two strains exhibited distinct phenotypic, physiological and genotypic characteristics that differentiate them from other related type species of the genus Altererythrobacter and related species in the family Erythrobacteraceae. Cells of the two strains were aerobic, Gram-stain negative, non-motile and rod-shaped. Optimal growth conditions for EGI L300112T and EGI L300120 occurred on marine agar 2216 at pH 7 at 30 °C. The major respiratory quinone was Q-10. The detected polar lipids of two strains included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and unidentified phospholipids. The major fatty acids (>10%) were identified as summed feature 3 (C16 : 1ω7 c/C16 : 1ω6 c) and summed feature 8 (C18 : 1ω7 c). The G+C content of strain EGI L300112T and EGI L300120 was 61.2% and 61.4%, respectively. Based on differential phenotypic and genotypic characteristics of the two strains and related species in the genus Altererythrobacter, the two strains should be classified as representing a new species of this genus, for which the name Altererythrobacter chaiwopuensis sp. nov. is proposed. The type strain is EGI L300112T (=MCCC 1K09432T=KCTC 8712T).
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