Aug 2026· Journal of Agricultural and Food Chemistry· Vol 74 33, pp.
25908-25922
· 0 citations· 44 references
Medicine
TL;DR
Results indicate that B. velezensis B05 has potential as a multifunctional biocontrol agent for sustainable management of soybean root rot.
Abstract
Soybean is an important global crop, but its production is often limited by root rot, which causes severe yield and quality losses. In this study, a potent antagonistic bacterium, Bacillus velezensis B05, was isolated from the rhizosphere of healthy soybean plants. Genome analysis identified eight biosynthetic gene clusters, including bacilysin and macrolide H, as well as 34 carbohydrate-active enzymes, suggesting its biocontrol potential. Metabolomic analysis detected 621 metabolites, and KEGG enrichment results were consistent with the genomic predictions. The cell-free supernatant of B05 significantly inhibited the growth of Fusarium spp. by 65.85%, spore germination by 75.85%, and disease incidence by 62.5%. Scanning electron microscopy revealed severe damage to fungal cells, including spore deformation and hyphal disintegration. In addition, B05 produced lytic enzymes and siderophores related to plant growth promotion. These results indicate that B. velezensis B05 has potential as a multifunctional biocontrol agent for sustainable management of soybean root rot.
B. velezensis BJ-1141 is a multifunctional bacterial strain with strong biocontrol efficacy and plant growth-promoting traits, supported by genomic evidence, and represents a promising candidate for sustainable crop disease management.
Yunyun Chen, Mei Liu, Wei Zhang et al.· Journal of Applied Microbiol...· 0 citations
Tomato cultivation worldwide faces problems with bacterial wilt and Fusarium wilt, which cause significant yield losses. This study evaluated Bacillus sp. SNRUSA4, a polylactic acid (PLA)-degrading bacterium isolated from compost, for plant growth promotion and biocontrol against these diseases. In vitro assays confirmed that SNRUSA4 effectively inhibited Ralstonia solanacearum and Fusarium oxysporum f. sp. lycopersici, the pathogens responsible for bacterial wilt and Fusarium wilt, respectively. Important traits, including phosphate solubilization, nitrogen fixation, biofilm formation, and the production of siderophore, indole-3-acetic acid, ammonia, and hydrolytic enzymes (amylase, cellulase, lipase, protease, and chitinase), which are consistent with its PLA-degrading capability, were observed in SNRUSA4. These traits contribute to both plant growth promotion and disease suppression. The seedling tray experiment indicated that the combined application of SNRUSA4 and L-tryptophan synergistically promoted tomato seedling growth, enhancing shoot and root lengths. Seed priming with SNRUSA4 enhanced tomato seedling growth as shown in fresh weight, dry weight, and vigor index. SNRUSA4 demonstrated efficacy in the biocontrol of bacterial and Fusarium wilt diseases, along with promoting tomato growth in pot experiments. Thus, this study highlights the potential of Bacillus sp. SNRUSA4 as a multi-functional strain for bridging bioplastic waste management with tomato growth promotion and disease control.
Suwapha Sawiphak, Aroon Wongjiratthiti· Anais da Academia Brasileira...· 0 citations
Quinoa leaf spot is an important fungal disease that limits quinoa yield and quality. This study aimed to evaluate the pathogenicity of isolates associated with quinoa leaf spot in Qinghai, China, and to screen multifunctional biocontrol bacteria with potential for further development. Pathogenicity assays conducted on healthy quinoa leaves showed that isolates Alternaria alternata AF15 and A. tenuissima AF18 induced typical leaf spot symptoms. The corresponding fungi were successfully re-isolated from the resulting lesions, confirming the pathogenicity of both isolates. Two highly effective biocontrol bacteria, Serratia liquefaciens CB82 and Bacillus velezensis CB316, were subsequently selected through dual-culture assays. Their maximum inhibition rates against the two fungal pathogens reached 56.00% and 57.00%, respectively. Both biocontrol strains exhibited broad adaptability to different temperatures, pH, and NaCl conditions, produced protease, amylase, and cellulase, and showed phosphate-solubilizing activity. Metabolite extraction and fractionation revealed that the antifungal substances were predominantly enriched in the n-butanol fractions, which caused severe shrinkage, surface roughening, breakage, and deformation of the pathogen hyphae. In addition, both strains exhibited strong biofilm-forming capacity and successfully colonized quinoa leaves. Their culturable populations peaked on day 3 after inoculation, reaching 5.58 × 107 and 6.06 × 107 CFU/mL, respectively. In seed germination pouch assays, the bacterial suspensions promoted quinoa root elongation, whereas the fermentation broths increased seedling biomass accumulation in pot experiments. Overall, this study confirmed the pathogenicity of fungal isolates associated with quinoa leaf spot in Qinghai, China, identified two promising biocontrol bacterial strains, and preliminarily characterized their antifungal substances. These findings provide valuable microbial resources and a research basis for the future development of biological control strategies against quinoa leaf spot.
The soil-borne oomycete pathogen Phytophthora capsici poses a serious threat to plant growth and results in substantial economic impact on the pepper industry. Therefore, the isolation of multifunctional biocontrol agents has become a major research priority. In this study, we isolated a biocontrol strain, Bacillus subtilis Z-294, which demonstrated strong inhibition of P. capsici in both plate and greenhouse experiments. This strain Z-294 exhibited broad-spectrum antagonistic activities as well as plant growth-promoting ability. Phylogenetic analysis based on core-genome and average nucleotide identity (ANI) further identified Z-294 as B. subtilis. Importantly, we found that the strain Z-294 alleviates the P. capsici disease through mechanisms associated with changes in the plant microbiome. Plants treated with Z-294 showed higher alpha-diversity. The application of B. subtilis Z-294 showed that the relative abundance of Alphaproteobacteria, Bacilli, Bacteroidia, Agaricomycetes, and Eurotiomycetes increased, while the relative abundance of Gammaproteobacteria and Sordariomycetes declined. Strain Z-294 holds great promise as a multifunctional biocontrol agent for the sustainable management of diseases and the enhancement of plant health, laying a solid foundation for its future agricultural applications.
Yanan Zhao, Qingchao Zeng, Kunzhi Long et al.· Agronomy· 0 citations
Abstract Soil-borne fungal pathogens significantly limit wheat production in Kazakhstan, particularly Fusarium solani (Mart.) Sacc. 1881 and Bipolaris sorokiniana (Sacc.) Shoemaker, 1959, which cause root rot and early seedling decline. This study aimed to isolate and characterize indigenous rhizosphere antagonists with biocontrol potential against these pathogens and to evaluate their effects on wheat seed germination and early growth. More than 20 microbial isolates were obtained from the rhizosphere of winter wheat. Two active antagonistic strains were selected by dual-culture assays: Bacillus sp. NK1 and Trichoderma harzianum S1. Inhibition zones against F. solani reached 23.75 ± 0.67 mm for NK1 and 32.89 ± 0.67 mm for S1. Cell-free culture supernatants significantly reduced mycelial growth and completely inhibited spore germination. Molecular identification based on 16S rRNA sequencing and TEF-1α/RPB2 analysis confirmed the taxonomic affiliation of both isolates. Seed treatment significantly improved germination (88%), root length, shoot length, and seedling vigor compared with untreated controls (P < 0.01). In pot experiments, both strains promoted biomass accumulation and improved seedling growth under pathogen pressure, with the Bacillus strain showing the strongest growth-promoting effect. Combined inoculation did not result in a synergistic response. These findings highlight the potential of indigenous rhizosphere microorganisms as biological control agents for the management of wheat root rot pathogens.
N. Kuldybayev, A. Sadanov, G. Baimakhanova et al.· Brazilian Journal of Biology· 0 citations
Aim: This study aimed to examine the biocontrol potential of plant growth-promoting rhizobacteria previously isolated from the tomato rhizosphere against Fusarium oxysporum in pepper plants.
Place and Duration of Study: This study was conducted at the Microbiology Laboratory of the Federal University, Oye-Ekiti, between July 2025 and May 2026.
Methods: Isolates were obtained from stock cultures and screened for key plant growth-promoting traits, including ammonia, siderophore, nitrogen fixation, and amylase production. Phytopathogenic fungi were isolated from apparently diseased tomato plants. The antifungal activity of the rhizobacterial isolates was evaluated using a dual-culture method. The greenhouse experiment was conducted using a completely randomised block design.
Results: Each of the five bacterial isolates exhibited important plant growth-promoting characteristics. Throughout the investigation, isolate IS4A produced the greatest increase in plant height, reaching more than 35 cm by Week 8. By Week 8, isolate IS9 had reached approximately 30 cm, representing the second-highest growth promotion. The highest percentage inhibition of Fusarium was recorded for isolate IS14B (56%), followed by IS13 (47%), IS4A (45%), IS9 (27%), and IS10 (24%). Among all groups, pathogen-inoculated plants (Control 1: Fusarium only) had the lowest fresh weight (5.21 g) and dry weight (0.42 g). IS9 recorded the greatest biomass accumulation, with a fresh weight of 32.12 g and a dry weight of 8.16 g, more than three times the healthy baseline (Control 2: 9.39 g fresh weight and 2.38 g dry weight).
Conclusion: The present findings indicate that Pseudomonas lactis and Bacillus tropicus have potential as biological control agents against Fusarium oxysporum while simultaneously promoting pepper growth.
O. Oyawoye, K. J. Ayantola, E. A. Omotoso et al.· International Journal of Pat...· 0 citations
