Significant advances in integrated strategies, particularly organelle engineering in Yarrowia lipolytica and Saccharomyces cerevisiae, have increased squalene titers to high levels and provide a useful basis for future metabolic engineering efforts aimed at improving squalene production and facilitating the synthesis o...
Saccharomyces cerevisiae is a widely used biomanufacturing chassis; however, its inherent Crabtree effect severely limits energy yield and diverts carbon flux away from the desired non-ethanol products. To address this challenge, we developed a multidimensional metabolic engineering strategy to alleviate the Crabtree e...
Shuyan Tang, Wenzhuo Gao, Jia-Ying Wang et al.· Metabolic Engineering· 1 citation· ⚡1
Oleanane-type pentacyclic triterpenoids are a class of naturally occurring specialized metabolites with diverse biological activities. Conventional plant extraction and chemical synthesis are constrained by low yields, high costs, and a reliance on natural resources. With the development of synthetic biology and metabo...
Fu-Yan Yuan, De-Yue Diao, Shi-Xiang Peng et al.· Journal of Agricultural and...· 0 citations
This review treats the isopentenol utilization pathway as a configurable precursor module rather than a single pathway variant, and identifies the regimes, and the quantitative margins, within which an alcohol-fed bypass outperforms further MVA or MEP optimization.
Y. Kim, Youngwoo Na, Won Yoon Hwan Choi et al.· Biotechnology Advances· 0 citations
Polyketides are among the most structurally diverse and therapeutically important classes of natural products, serving as antibiotics, anticancer agents, agrochemicals, and industrial pigments. Their structural complexity and limited natural availability have driven the development of microbial biosynthetic platforms a...
Lycopene is a high-value carotenoid widely utilized in food, pharmaceutical, nutraceutical, and cosmetic industries because of its strong antioxidant properties and associated health benefits. Among microbial hosts, Saccharomyces cerevisiae has emerged as a promising platform for sustainable lycopene biosynthesis due t...
Farah D. Garaad, H. Karaca· Frontiers in Bioengineering...· 0 citations
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