Aug 2026· Biosaintifika: Journal of Biology & Biology Education· Vol 18, pp. 233-240· 0 citations
Abstract
Shallot (Allium ascalonicum L.) is a major agricultural commodity in Indonesia. This plant contributes significantly to the national economy and contains numerous bioactive compounds with various properties, such as antioxidant, antibacterial, and antifungal activity. However, the biggest production constraint is infection by the pathogen Fusarium, known as Twisting disease. Therefore, this study aimed to investigate the effect of combining biopesticides and liquid organic fertilizer (LOF) in shallot farming on Fusarium spp. The experiment was conducted using randomize block design with two main parameters. The first parameter was the interval of biopesticide extract applications, namely, no extract, once a week, and once every two weeks. The second parameter was the LOF dosage, including 0, 25, 50, and 75 mL/plant. Plant height, number of leaves, fresh biomass, and disease incidence were observed to evaluate the effect of biopesticides and LOF application. The results showed that a combination of biopesticide extract and LOF positively affected growth but suppressed disease incidence. The combination of once-a-week biopesticide extract and 75 mL/plant LOF applications was identified as the best treatment. In conclusion, biopesticide extract and LOF can be applied for sustainable, environmentally friendly disease management in shallot plants. The results support Sustainable Development Goals (SDGs) 2, 12, and 15 by controlling Fusarium fungal infections in shallots, maintaining production, reducing the use of chemical pesticides, and promoting sustainable agricultural management.
Amaranth is a widely consumed traditional vegetable with high nutritional value. However, its production is constrained by both biotic and abiotic factors, which cause significant field damage. To address this issue, farmers often rely on a variety of pesticides, many of which are unregistered and pose risks to both humans and the environment. Hence, there is an urgent need to find alternatives to these synthetic products. In this context, the present study was undertaken to improve amaranth production using Kogle-zanga, a biopesticide. A completely randomized block design with four replications and four treatments was used. The biopesticide’s efficacy was assessed based on pest attack levels, plant growth and development, and leaf yield. The results showed that Kogle-zanga effectively controlled pests. The T3 dose (70 mL/ha) had a more favorable effect on growth, development, and leaf yield compared to the T1 (50 mL/ha) and T2 (60 mL/ha) doses. Among the tested doses, T3 (70 mL/ha) was the most effective. Additionally, the biopesticide enabled amaranth to achieve its potential yield of 1–2 t/ha. Therefore, promoting the use of this biopesticide among producers for amaranth leaf cultivation is essential to protect human health and the environment.
Drabo Samuel Fogné*, Nikiéma Dominique· International Journal of Bio...· 0 citations
Seed treatment is an effective strategy for improving seed vigor, plant establishment, and crop productivity. However, information regarding the effectiveness of peptide-based seed treatment methods on shallot (Allium ascalonicum L.) remains limited. This study evaluated the effects of different seed treatment methods using Golden Grow and keratin hydrolysate peptide on the growth and yield of shallots. The experiment was conducted in Songan B Village, Kintamani, Bali, Indonesia, using a randomized block design with three seed treatment methods and nine replications. The treatments consisted of: (Sa) farmers' conventional practice by cutting one-third of the bulb tip, (Sb) coating bulbs with a solution containing 1 mL Golden Grow L⁻¹ water and 2 mL peptide L⁻¹ water, and (Sc) briefly dipping bulbs in the same solution before planting. Growth variables, physiological traits, yield components, and bulb productivity were analyzed using analysis of variance followed by the least significant difference test at the 5% level. The conventional cutting treatment (Sa) produced superior vegetative growth, resulting in the highest leaf chlorophyll content (74.58 SPAD units), relative water content (87.96%), and number of leaves (53.74). In contrast, the coating treatment (Sb) consistently produced the highest yield performance, including bulb weight per clump (118.93 g), number of bulbs per clump (18.65), yield per plot (4.28 kg), and estimated yield per hectare (14.27 t ha⁻¹). These findings indicate that coating shallot bulbs with Golden Grow and keratin hydrolysate peptide is a promising seed treatment approach for enhancing yield while maintaining favorable plant growth, offering an environmentally friendly alternative for sustainable shallot production.
N. Naomi, I. Rai, Ni Nyoman Ari Mayadewi· Magna Scientia Advanced Biol...· 0 citations
Kailan (Brassica oleracea var. alboglabra) is a leafy vegetable with high economic value whose productivity is strongly influenced by nutrient availability and growing media conditions. Eco-enzyme fertilizer has emerged as a promising environmentally friendly organic fertilizer that may enhance plant growth through the improvement of nutrient availability and biological activity in the growing medium. This study aimed to evaluate the effectiveness of different eco-enzyme fertilizer concentrations in improving the growth of kailan. The research was conducted at the experimental field in Titigalar Hamlet, Baturiti District, Tabanan Regency, Bali, Indonesia. The experiment was arranged in a randomized complete block design (RCBD) with one factor consisting of four eco-enzyme fertilizer concentrations: 0, 50, 100, and 150 mL L⁻¹ of water. Each treatment was replicated three times, resulting in 12 experimental units. The observed variables included plant height, number of leaves, leaf area, and leaf chlorophyll content. Data were analyzed using analysis of variance (ANOVA), followed by Tukey’s Honestly Significant Difference (HSD) test at the 5% significance level. The results showed that eco-enzyme fertilizer application had a highly significant effect on all observed growth parameters, including plant height, number of leaves, leaf area, and leaf chlorophyll content. Increasing eco-enzyme concentrations enhanced vegetative growth compared with the untreated control. The application of eco-enzyme at 100 mL L⁻¹ produced the most favorable vegetative growth, as indicated by superior plant height, leaf number, leaf area, and chlorophyll content. These findings demonstrate that eco-enzyme fertilizer is effective in promoting kailan growth and has considerable potential as an environmentally friendly organic fertilizer to support sustainable kailan cultivation.
Beriselamat Gea, Ni Luh Kartini, N. M. Trigunasih· International journal of mul...· 0 citations
Abstract The soil used is sandy in texture, low in nutrients, and low in organic matter, and therefore has low cation exchange capacity. Therefore, the objective was to evaluate the effectiveness of improving their physicochemical and biological properties by inoculating the roots with a solution of Methylobacterium and liquid compost in cauliflower cultivation, as well as integrating a treatment through an automated drip irrigation system. This biostimulant, biofertilizer, and biocontrol agent solution was used, which has applications in agriculture to improve soil health and crop productivity. The experiment was set up under a completely randomized block design with four treatments (T0, T1, T2, and T3), corresponding to doses of 0, 250, 333, and 400 mL per 200 L of water, respectively. The results indicated that treatment T3 significantly optimized the plant's physiological response to salt stress, increasing both its antioxidant capacity and chlorophyll α concentration. Likewise, physical characterization revealed significant differences in the morphological parameters of cauliflower, suggesting greater metabolic resistance and improved nutritional quality. Finally, ultrastructural analysis of the epidermis and stomata using microscopy showed that, while the control treatment (T0) had a collapsed surface, treatment T3 showed a functional and turgid ultrastructure. This demonstrates that the inoculation applied mitigates the phytotoxic impact of the substrate and optimizes the metabolic potential of the crop.
J. A. Legua Cárdenas, E. Macavilca Ticlayauri, M. S. Sánchez Calle et al.· Brazilian Journal of Biology· 0 citations
Global demand for horticultural commodities, particularly Bird’s Eye Chili (Capsicum frutescens L.), continues to increase due to their function as a household food ingredient and a source of bioactive compounds with health benefits. In Indonesia, Capsicum frutescens are a strategic commodity that influences inflation, making increasing productivity a crucial part of national food security. However, low soil fertility and high dependence on inorganic chemical fertilizers hamper productivity while increasing production costs and environmental pressures. Sustainable fertilization using local organic resources is an important strategy to improve soil health and plant performance. This study aimed to evaluate the combined effect of liquid organic fertilizer (LOF) and Azolla microphylla compost on Capsicum frutescens growth and yield, by simultaneously assessing five parameters: plant height, stem diameter, number of leaves, number of branches, and fruit production. The experiment was conducted in Langgomea Village, Konawe, Southeast Sulawesi, with chili seedlings planted in 25 cm polybags containing a mixture of local garden soil and rice husk charcoal (2:1 v/v). A factorial Randomized Complete Block Design was applied with LOF (0, 10, 20, 40 ml/L) and compost (0, 10, 20, 40 g/plant), resulting in 16 treatment combinations, each replicated three times (a total of 144 plants). Growth and yield data were recorded weekly for 12 weeks; harvesting was carried out from the first to the last harvest. Statistical analysis using ANOVA and LSD at a significance level of 5% with R 4.3 and SPSS 28, provides a scientific basis for sustainable organic fertilization strategies and optimization of local resources. The results showed that the combination of LOF and Azolla microphylla compost significantly affected all growth and production parameters of Capsicum frutescens. The highest plant height was achieved in the P3K3 treatment (83.67 cm), followed by an increase in stem diameter to 136 mm, number of leaves to 104, and number of branches to 31.33. This increase in vegetative growth is in line with the high nitrogen and phosphorus content of Azolla, which supports cell formation, chlorophyll, protein, and nucleic acid synthesis. It was also found that Capsicum frutescens L. fruit production increased significantly with the combination of LOF and compost, reaching the highest average of 108.67 kg per plant in the P2K3 treatment. This confirms that the application of Azolla-based organic fertilizer provides sufficient nutrients, increases photosynthesis, biomass, and fruit yield accumulation. The interaction of liquid fertilizer and compost provides optimal synergistic effects for vegetative growth and plant productivity in the field. These findings bridge the research gap regarding the simultaneous use of liquid fertilizer and Azolla compost on Capsicum frutescens L., in humid tropical agroecosystems, while also providing practical implications for sustainable organic fertilization strategies. Application of these findings can improve resource efficiency, reduce dependence on chemical fertilizers, and support environmentally friendly tropical horticultural management.
Keywords: Bird’s Eye Chili, liquid organic fertilizer, Azolla microphylla compost, fruit yield, humid tropical agroecosystem, sustainable fertilization.
S. Suharjo, Ulyasniati Ulyasniati· Journal of Global Innovation...· 0 citations
This study aimed to evaluate the effects of natural extracts and Burkholderia spp. bacterial strains on the growth, yield, and quality of lettuce (Lactuca sativa cv. Muir) under greenhouse conditions. The experiment was conducted using a completely randomized block design with two factors: (A) two natural extracts (catfish waste extract FE and soybean oil cake extract SE), and (B) three bacterial strains (MP1, MP2, MP3), and no bacterial strain. A total of 8 treatments were set up, with 6 replications per treatment. Agronomic parameters were recorded at 15, 35, and 55 days after sowing (DAS) to evaluate the growth-promoting effects of the extracts and bacterial strains on Muir lettuce. The yield components and quality parameters were determined at 55 DAS. The results demonstrated that the FE extract was more effective than the SE extract (p<0.01), enhancing plant growth, producing marketable head weights of 300 g.plant-1 and 287 g.plant-1, respectively. Similarly, the MP1 strain significantly enhanced the growth and the marketable head weight. The application of natural extracts with bacterial strains reduced postharvest weight loss to below 5% after a 15-day storage period...
T. Pham, Trinh Nguyen Truong, Thi Bich Thuy Vo et al.· CTU Journal of Innovation an...· 0 citations