Jul 2026· ACS Applied Bio Materials· 0 citations· 50 references
Medicine
TL;DR
In vivo diabetic mouse wound models confirmed that the MSFP hydrogel synergistically improved the wound microenvironment by establishing barrier protection, inhibiting bacterial growth, reducing inflammation, promoting M2 macrophage polarization, and enhancing vascularization.
Abstract
The abnormal microenvironment of chronic diabetic wounds leads to the disorder of the tissue repair process. Herein, based on the principles of click chemistry, a multifunctional rapid gelation hydrogel dressing (MSFP hydrogel) was designed for the treatment of diabetic wounds. The hydrogel was formed via thiol-ene click reaction between thiolated Pluronic F127 (F127SH) and maleimide-modified gelatin (GelatinMAL). This thiol-ene click reaction required no metal catalyst, exhibited good biocompatibility, and proceeded under mild conditions. The system incorporated antimicrobial component F127-acetylbromide (F127AcBr) and puerarin (PUE) and responded to overexpressed matrix metalloproteinase 9 (MMP-9) at wound sites, enabling controlled drug release. In vitro experiments demonstrated that the MSFP hydrogel effectively eliminated ROS; modulated macrophage polarization; promoted human umbilical vein endothelial cell proliferation, migration, and tube formation; and exhibited robust antibacterial activity against Staphylococcus aureus and Escherichia coli. In vivo diabetic mouse wound models confirmed that the MSFP hydrogel synergistically improved the wound microenvironment by establishing barrier protection, inhibiting bacterial growth, reducing inflammation, promoting M2 macrophage polarization, and enhancing vascularization. Collectively, this multifunctional hydrogel can promote diabetic wound healing through immunoregulation, angiogenesis, and antibacterial activity, presenting a promising therapeutic strategy for clinical application.
Diabetic chronic wounds remain a major clinical challenge due to persistent infection, excessive inflammation, and impaired tissue regeneration. Herein, we report a multifunctional peptide-based antibacterial hydrogel (PAHG) for treating infected wounds, constructed through the incorporation of Cys-Arg-NH2 (CR)-Ag nanoassemblies into a bioactive matrix. In this design, the CR dipeptide enables in situ reduction and stable coordination of silver ions, yielding CR-Ag nanoassemblies with controlled silver release and enhanced biocompatibility. Co-assembly of this antibacterial component with chitosan, gelatin, collagen, and epidermal growth factor (EGF) affords a three-dimensional hydrogel network that provides a moist wound microenvironment, structural support, and sustained release of pro-regenerative cues. The resulting PAHG system exhibits excellent antibacterial activity against Escherichia coli and methicillin-resistant Staphylococcus aureus, with inhibition efficiencies exceeding 80%, while maintaining high cytocompatibility with human skin fibroblasts (∼150% viability). In a diabetic infected wound model, PAHG significantly accelerates wound healing, achieving nearly complete closure within 12 days, accompanied by enhanced collagen deposition and no noticeable histopathological abnormalities in major organs. By integrating molecularly engineered antibacterial nanoassemblies with rationally designed multicomponent hydrogels, this work provides a promising strategy for the development of bio-based antimicrobial materials and chronic wound dressings.
Heng Ge, Wen Yuan, Yulin Sun et al.· Journal of materials chemist...· 0 citations
It is demonstrated that the catechol-nanocellulose/chitosan polymeric hydrogel effectively overcomes adhesion, infection, and oxidative stress barriers in diabetic wound healing, making it a promising candidate for difficult-to-heal chronic ulcers.
With its integrated hemostatic, antioxidant, antibacterial, and pro-regenerative properties, the CBOS hydrogel offers a viable and attractive therapeutic approach for complex wound tissue repair.
Xueyan Hou, Yanan Lu, Tenglong Xu et al.· ACS Applied Materials and In...· 0 citations
HA-c-FZ1 functions as a pH-responsive hydrogel dressing that combines peptide delivery and antimicrobial, redox-regulatory, immunomodulatory, and pro-angiogenic functions that positions it as a therapy for chronic diabetic wound repair by simultaneously addressing infection, inflammation, oxidative stress, and vascular regeneration.
Zhe Fu, Jingyu Jiang, Yutong Wu et al.· Burns & Trauma· 2 citations
Chronic diabetic wounds remain a major clinical challenge owing to persistent bacterial infection, prolonged inflammation, excessive exudation, and impaired tissue regeneration. Herein, an injectable thermosensitive hydrogel was developed by integrating N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride with aldehyde-functionalized Pluronic F127 for epidermal growth factor (EGF) delivery and diabetic wound repair. The hydrogel forms a dual-crosslinked network through temperature-induced micellization and dynamic Schiff base bonding, exhibiting rapid gelation under physiological conditions, shear-thinning behavior, and self-healing properties. In vitro, the hydrogel provides a sustained release profile of EGF exhibiting effective antibacterial activity against Gram-positive S. aureus. In vivo studies in streptozotocin-induced diabetic rats demonstrate significantly accelerated wound healing, achieving 83% wound closure within 14 days compared to 45% in the control group, along with enhanced tissue regeneration characteristics, including improved collagen deposition. This multifunctional hydrogel provides a promising strategy for diabetic wound management by integrating antibacterial potential and tissue regeneration.
The high glycemic environment of diabetic wounds predisposes to bacterial infections that seriously threaten the wound healing process. Therefore, inhibition of bacterial infection and remodeling of the wound microenvironment are crucial for the healing of diabetic infected wounds. In this study, gold nanoparticles (Au NPs) and glucose oxidase (GOx) were loaded in situ onto the surface of zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) to prepare ZIF-8@Au@GOx (ZAG) nanoenzymes. It catalyzed the production of glucuronic acid and H2O2 from glucose, lowered the wound pH, promoted the release of Zn2+, and catalyzed the production of hydroxyl radicals (·OH) from H2O2, thus serving to regulate the wound microenvironment, reducing glucose levels, and providing efficient antimicrobial activity. Subsequently, the nanoenzymes were loaded into β-cyclodextrin-based host-guest interaction and perylene-bonded tandem highly dynamic adaptive (DSA) hydrogels to obtain nanoenzyme-functionalized hydrogels (ZAG@Gel). The hydrogel could adapt to the shape of the wound, effectively fit the damaged skin, and play a role in reducing the toxicity and slowing down the release. In vitro studies showed that ZAG@Gel could release ZAG nanoenzymes in situ, causing the bacterial surface to crumple, the cell membrane structure to be disrupted, and the internal substances to be leaked. In vivo animal experiments showed that the wound healing rate after ZAG@Gel treatment reached nearly 98.06%. Thus, this highly effective antibacterial nanoenzyme-functionalized hydrogel has a strong bactericidal ability and shows good pro-diabetic infected wound healing properties.
Changyu Yao, Dongqin Zhang, Jiaqi Kan et al.· ACS Biomaterials Science & E...· 0 citations