Isolation of Antibiotic Resistant Bacteria from Bichitrapur Mangrove forest of Odisha and Identification of factors conferring Antimicrobial Resistance
TL;DR
Insight is provided into the genetic targets, their resistance mechanism and potential metabolites that can be exploited for discovery of new drugs.
Abstract
The antimicrobial resistant microbes possess resistance genes as well as synthesize various secondary metabolites which protect them from extreme environmental conditions. Isolating microbial strains from extreme environments like mangrove forest increases the possibility of isolating antimicrobial compounds that have not been previously isolated and secondly, because the pathogens are not exposed to these bacterial compounds, it is unlikely that they will develop antibiotic resistance to the bacteria. The present work encompasses isolation of antibiotic resistant microbes from mangrove forest which has less anthropogenic activity. Culture based method was adapted to isolate strain FMU-55 which was identified to be Stappia indica using 16s rRNA sequencing. In silico approach using CARD-RGI led to the identification of adeF gene that belongs to the RND (Resistance-nodulation-cell-division) and qacJ gene belonging to small multidrug resistance (SMR) antibiotic efflux pump family. Simultaneous metabolomics studies using GCMS led to identification of various antimicrobial metabolites, like N,NDiethylaniline, Oxime-methoxy-phenyl-, Cycloheptasiloxane, Tetradecamethyl, Phenol, Ethanone, 1,1'-(2,6-dimethyl-3,5-pyridinethiol) and Tert-Butyldimethylsilyl trifluoromethanesulfonate. This study provides insight into the genetic targets, their resistance mechanism and potential metabolites that can be exploited for discovery of new drugs.