Combining ability and gene action for grain yield and biofortification traits in pearl millet [Pennisetum glaucum (L.) R. Br.]: implications for breeding high-yielding biofortified hybrids in arid regions

Abstract

Hybrid RIB-9184 × RIB-15131 combines high yield (18.84 g plant⁻¹) with iron (46.16 mg kg⁻¹), zinc (38.86 mg kg⁻¹), and protein (11.91%); Fe–Zn correlation (rg = 0.82) permits simultaneous biofortification. Pearl millet [Pennisetum glaucum (L.) R. Br., syn. Cenchrus americanus (L.) Morrone] is a climate-resilient cereal with inherently high micronutrient levels, making it a priority crop for biofortification. Understanding gene action for yield and nutritional traits is essential for designing effective breeding strategies. Ten diverse inbred lines were crossed in a half-diallel design (Griffing’s Method 2, Model 1), and the 55 entries (45 F1 hybrids + 10 parents) were evaluated across two sowing-date environments in a randomised complete block design with three replications at Jaipur, Rajasthan, India. Biofortification traits (Fe, Zn, protein) showed predominantly additive gene action (Baker’s ratio 0.71−0.91) with high heritability (0.90−0.94). G×E interaction was significant for Fe and Zn but genotypic variance was substantially larger, maintaining high heritability; protein showed no G×E interaction. Grain yield was governed largely by non-additive effects (Baker’s ratio 0.54) with significant G×E interaction, favouring hybrid breeding. Among parents, RIB-9205 had the highest GCA for Fe (6.65, P < 0.001), RIB-9184 for Zn (3.85, P < 0.001) and protein (0.78, P < 0.001), and RIB-9185 was a balanced combiner for yield (1.39, P < 0.001) and micronutrients. The hybrid RIB-9184 × RIB-15131 ranked first across all five weighting schemes of the multi-trait performance index (1.31), combining grain yield of 18.84 g plant⁻1 with Fe of 46.16 mg kg⁻1, Zn of 38.86 mg kg⁻1, and protein of 11.91%. The strong Fe−Zn correlation (rg = 0.82, P < 0.01) permits simultaneous micronutrient improvement. An integrated approach combining hybrid development for yield with population improvement for micronutrient density is recommended for biofortified pearl millet cultivars in arid regions.