Hepatoprotective Effects of Dulaglutide against Methotrexate-Induced Liver Injury in Rats

Abstract

Background and Objectives: The cellular antioxidant defense system is disrupted, which results in hepatotoxicity. When the antioxidant defence system is weakened, hepatocyte oxidative damage results from an increase in reactive oxygen radicals. The chemotherapeutic and immunosuppressive drug methotrexate (MTX) is often linked to hepatotoxicity, a serious side effect with uncertain consequences. Therefore, the current study looks into the potential preventive effect of dulaglutide (DUL) against liver damage caused by MTX. Methods: The experiment included forty adult male wistar rats, who were divided into four groups: Group 2 was given 14 mg/kg/week of oral MTX for two weeks. On top of the oral MTX, Group 3 also got 0.1 mg/kg/week of subcutaneous DUL. Distributed under the skin, Group 4 was given 0.1 mg/kg/week of DUL. One group served as a control. The animals were sacrificed on the fifteenth day so that histopathological and biochemical testing could be carried out. In these investigations, liver function tests (ALT and AST) and markers of oxidative stress (MDA, GSH, CAT, and SOD) were included.. Results: Our findings showed that MTX significantly raised a number of oxidative stress markers and liver function tests, employing hematoxylin and eosin (H&E) staining to support biochemical data with histological findings. Conclusions: Due to its antioxidant qualities and liver function, DUL significantly protects against MTX-induced hepatotoxicity. This demonstrates its medicinal potential in avoiding hepatotoxicity caused by drugs