[Multidimensional metabolic engineering of Yarrowia lipolytica for β-carotene production].

[Metabolic engineering of Yarrowia lipolytica for astaxanthin production].

Systematic metabolic engineering of Yarrowia lipolytica for highly efficient biosynthesis of mannitol from glucose.

Mannitol is widely utilised in the food and pharmaceutical industries. Current commercial production primarily depends on chemical synthesis, a process that is expensive, environmentally detrimental, and requires high-temperature/pressure as well as complex purification steps. While microbial production of mannitol has...

Advances in Polyketide Related Biosynthesis Using Engineered Yarrowia lipolytica

This review summarizes recent progress in PKS pathway engineering in Y. lipolytica, covering the biosynthesis and optimization of representative type I and type III polyketides, including 6-MSA, TAL and the unnatural polyketide HHEP.

Metabolic Engineering of Saccharomyces cerevisiae for Flavonoid Synthesis from Acetate

Acetate is a sustainable C2 feedstock derived from CO2 electroreduction, but its conversion to complex natural products is constrained by impaired cell growth and poor metabolic compatibility. Adaptive laboratory evolution generated an evolved Saccharomyces cerevisiae strain capable of producing 536.32 mg/L of p-coum...

Efficient Production of Vanillyl Alcohol in Escherichia coli via Protein and Pathway Engineering.

Vanillyl alcohol (VNA) is a high-value compound used in the pharmaceutical industry and other fields. Its microbial de novo biosynthesis is severely limited by the low catalytic efficiency of O-methyltransferase. Here, we constructed a V314I mutant of caffeate O-methyltransferase via structure-guided protein engineerin...

Menaquinone-7 production by engineered Saccharomyces cerevisiae through de novo Synthesis and fermentation optimization