Vitamin B 1 ‐Catalyzed Green Synthesis of Thiazole Derivatives and Their Anticancer Activity Against MOC2 Cells

Abstract

An efficient and simple one‐pot synthetic protocol has been developed for the first time to synthesize the series of (2‐bromophenyl)‐(4‐phenylthiazol‐2‐yl) amines. This was achieved by the condensation of 1‐(2‐bromophenyl) thiourea and substituted phenacyl bromide in the presence of the catalytic amount of vitamin B 1 as a readily available, water‐soluble, reusable, and biodegradable catalyst. This technique is a very promising asset; it provides mild reaction conditions, an environmentally benign, greener approach, an easy workup process, high yield, less reaction time, and low cost. Structural confirmation was achieved through 1 H NMR, 1 3 C NMR, and LC–MS analysis. Among various heterocyclic scaffolds, the thiazole ring has attracted considerable attention due to its broad spectrum of pharmacological activities, including anticancer. MOC2 murine oral squamous cell carcinoma cells were used as an in vitro model, with the MTT assay serving as an initial screening method to evaluate the bioactivity of newly synthesized compounds by assessing cancer cell viability, comparative efficacy, and structure–activity relationships.