Vaginal microbiome metabolite shifts as early predictors of premature ovarian insufficiency in adolescents with novel gene variants

Abstract

Vaginal microbiome metabolite profiling is an integrated omics-based diagnostic framework that combines metabolic profiling with biochemical-functional remodeling to identify biochemical dysregulations associated with POI. This study leverages non-invasive collection of vaginal samples and advanced high-throughput methods, such as 16S ribosomal ribonucleic acid sequencing and liquid chromatography-mass spectrometry, to quantify metabolic fluxes and pathways. In POI, altered Lactobacillus predominance and specific metabolic derangements have been demonstrated, reflecting condition-specific microbial profiles. These integrated microbial-metabolite signatures enhance diagnostic accuracy beyond taxonomic profiling alone, suggesting strong predictive potential. Overall, this method is an emerging precision medicine strategy that integrates microbial and genetic data to advance early detection and therapeutic management of POI in at-risk youth (2)