Multi-omics reveals a host-microbe metabolic synergy underlying brown planthopper biotype divergence.

INTRODUCTION The brown planthopper (BPH) is a highly destructive pest in rice production. The widespread cultivation of resistant rice varieties drives BPH populations to evolve new biotypes, posing a continuous challenge to crop protection. Understanding the adaptation mechanisms of these biotypes is therefore critical.

Objectives

This study aimed to investigate the potential mechanisms underlying the adaptation of a new BPH biotype to resistant rice varieties.

Methods

We compared the host adaptability of Biotype 1 and Biotype Y on a resistant rice variety (carring Bph15). An integrative multi-omics approach (transcriptomic and metabolomic) was employed, alongside metagenomic analysis of symbiotic microbiota.

Results

Biotype Y exhibited superior adaptation to the resistant variety. Multi-omics analysis revealed that the adaptation was associated with upregulation of carbohydrate, detoxification, and energy metabolism pathways, downregulation of vitamin pathways, and a distinct symbiotic microbiota enriched in Wolbachia. Functional experiments provided evidence suggesting that Wolbachia may contribute to this enhanced adaptation, possibly through riboflavin supplementation.

Conclusion

The adaptation of BPH to resistant rice appears to be associated with coordinated transcriptional and metabolic reprogramming, potentially involving symbiont-mediated nutritional support. These findings may provide new insights into tripartite host-pest-microbiota interactions and offer potential targets for sustainable pest management strategies.