Protein corona influences cellular uptake and exocytosis of luminescent nanoparticles at various serum concentrations in culture medium

Tuning Bovine Serum Albumin Nanoparticle Properties Through Desolvating Agents and Synthesis Parameters: Implications for Proteolysis and Drug Release

Bovine serum albumin nanoparticles (BSA-NPs) are widely used as model protein nanocarriers, and their physicochemical properties are strongly influenced by synthesis conditions. This study evaluated the effect of four desolvating agents (DAs), namely acetone, ethanol, isopropanol, and acetonitrile, differing in polarit...

Serum Lipids Remodel the Biomolecular Corona to Direct Nanoparticle Biodistribution.

Biomolecular corona (BC) governs nanoparticle (NP) interactions with biological systems. While the role of proteins in BC regulation is well recognized, the contribution of serum lipids remains largely unexplored. Here, silica magnetic NPs were incubated with normal or hyperlipidemic serum to form BC@NPs, followed by l...

Investigation of Extracellular Vesicle Corona Formation with Label-Free Optical Waveguide Lightmode Spectroscopy Method

Extracellular vesicles (EVs) are lipid bilayer-delimited particles that are naturally released from all types of cells into the extracellular environment. During their release to the extracellular space, EVs adsorb biomolecules, collectively designated as EV corona. In this study, we developed a novel method for immobi...

Bottom-up engineering of EV-mimetic vesicles reveals selective protein loading and modulates cellular uptake and biodistribution

Liposome decoration via aminoalkyl nitrobenzene molecular gate triggers hypoxia-responsive cargo release.

Findings validate the developed manufacturing method and highlight 15% HR-LPs as a promising platform for further in vivo studies with antitumor agents, aiming to achieve controlled and targeted drug delivery in hypoxia-associated tumor environments.

Decoding extracellular vesicle-biomolecule interactions: How the biomolecular corona shapes extracellular vesicle biodistribution and clearance in vivo.

Extracellular vesicles (EVs) are promising endogenous nanocarriers, yet how biomolecules adsorption shapes their in vivo fate remains unclear. Here, we examine how biomolecular corona formation regulates the biodistribution, cellular recognition, and clearance of extracellular vesicles. Using EVs derived from macrophag...