Jul 2026· Archives of Phytopathology and Plant Protection· Vol 59, pp. 1094 - 1107· 0 citations· 20 references
Abstract
Abstract Tobacco mosaic virus (TMV) is a stable, mechanically transmitted Tobamovirus infecting solanaceous hosts, but complete-genome data from naturally infected wild hosts in Egypt remain limited. Symptomatic wild tree tobacco, Nicotiana glauca Graham, was collected from New Borg El-Arab, Alexandria Governorate, Egypt, and examined for TMV infection. Among 29 symptomatic samples screened by DAS-ELISA, two samples, S10 and S24, were positive. Both were confirmed by RT-PCR using movement protein-specific primers, which amplified the expected 422-bp fragment. Sample S10 was selected for single-lesion purification and complete-genome sequencing, and the resulting isolate was designated TMV isolate 30102. Biological indexing confirmed infectivity, and transmission electron microscopy revealed rigid rod-shaped particles approximately 300 nm long. The complete 6,395-nt genome was assembled from seven overlapping RT-PCR fragments and deposited as ON142174.1. Sequence identity and maximum-likelihood phylogeny placed ON142174.1 within the globally distributed TMV population.
Abstract Capulavirus betae (beet capulavirus 1, BCV1) is a recently described species of the genus Capulavirus (family Geminiviridae) that was first identified in sugar beet in France. Capulaviruses are new group of geminiviruses because they are transmitted by aphids. Here, we report the identification and molecular c...
Omid Eini, P. Margaria, D. Knierim et al.· Journal of General Virology· 0 citations
The combined serological, RT-PCR, Sanger sequencing, and ONT metagenomic results confirmed TRSV infection in symptomatic H. sabdariffa plants, the first report of TRSV infecting H. sabdariffa in the United States, expanding the known host range of this economically important virus.
M. R. Ribeiro-Junior, Jennifer D. Olson, Sara Wallace et al.· Plant Disease· 0 citations
The fungal pathogen Pyricularia oryzae is notorious for causing blast disease in various important cereal crops, including wheat, rice, millet, and oat. Whole-genome-informed data on this pathogen are necessary to better understand the host adaptability of the fungus, including identifying key determinants of infection...
Pedro Rosa, J. Bilro, R. S. Ramiro et al.· bioRxiv· 0 citations
This study represents the first reports of the complete genome characterization of TSWV and phytoplasma infection in China aster in India along with the development of sensitive qPCR, RT-LAMP, and duplex PCR assays for rapid detection of these pathogens providing valuable tools for disease diagnosis, epidemiological st...
S. N. Kumar, L. Monisha, G. S. Madhu et al.· 3 Biotech· 0 citations
Tetrastigma hemsleyanum
is a valuable and rare medicinal herb in the family
Vitaceae
. With its large‐scale cultivation in China, a virus‐like disease has emerged threatening both yield and quality in recent years. However, the viral pathogen has not been reported. Here, we detected a new isolate of Cymbidium mo...