Application of Pepper aptamer-based reporter system for bacterial transcription studies

Comparative Characterization of σ32-Dependent Promoters for the Heat-Inducible Expression of FAST-PETase in Escherichia coli

Efficient regulation of recombinant enzyme expression is an important consideration for the development of microbial biocatalysts. Heat-inducible promoters regulated by the alternative sigma factor σ32 provide an inducer-free strategy for controlling gene expression in Escherichia coli. In this study, four σ32-dependen...

Fluorescence-Based Assays to Evaluate the Functionality of Phage-Derived Regulatory Elements for Applications in Synthetic Biology.

Characterization of metabolite-sensing riboswitches using in vivo reporter gene assays.

Macroscopic and microscopic visualization of Pseudomonas syringae pv. tabaci infection using complementary bioluminescent and fluorescent reporter systems

The development of bioluminescent and fluorescent reporter systems has enabled visualization of plant-pathogenic bacterial infection dynamics, providing insights into detailed mechanisms of infection and potential applications for disease control. Pseudomonas syringae pv. tabaci 6605 ( Pta ) possesses quorum...

Targeting bacterial TPP riboswitches using pVEC-conjugated chimeric antisense oligonucleotides.

Transcriptional analysis of D, D-carboxypeptidase genes as secondary cephalosporin resistance reporters in Escherichia coli harbouring blaCTX-M.

The emergence of novel plasmid-encoded resistance variants complicates antimicrobial resistance detection by conventional PCR-based methods. Therefore, endogenous secondary resistance genes expressed under antibiotic stresses to produce adaptive resistance mechanisms in bacteria may serve as reporters for AMR detection...