Supplementary data and reproducibility files for: 5-Methoxytryptophan treatment is associated with behavioral, peripheral serotonin-immune, and central serotonin transporter availability patterns in a rat inflammatory pain model

Abstract

This record contains the final Supplementary Data workbook and reproducibility resources supporting the manuscript “5-Methoxytryptophan treatment is associated with behavioral, peripheral serotonin-immune, and central serotonin transporter availability patterns in a rat inflammatory pain model.” The study evaluated 5-methoxytryptophan in male Wistar rats with complete Freund’s adjuvant-induced inflammatory pain. The workbook contains RNA-seq sample metadata and a gene-symbol-collapsed count-like matrix from an RBC-lysed peripheral blood leukocyte fraction (n = 2 per group per time point; 22 samples in total); differential-expression, gene-set-enrichment, module-score, detectability, directional-opposition, and gene-membership tables supporting Figures 2–5; animal-level mechanical-threshold, paw-thickness, forced-swim, and sucrose-preference data, summaries, and statistical outputs supporting Figure 6; animal-level cross-sectional 4-[18F]-ADAM SERT-specific uptake-ratio values, summaries, pointwise statistics, CFA-to-Sham reference-scale estimates, and group-summary tables supporting Figure 7; and a sheet index, column dictionary, and software-version information. The accompanying archive contains the final Figure 2–7 generation scripts, required analysis input files, and R session-information records. The RNA-seq samples represent a mixed RBC-lysed peripheral blood leukocyte fraction, and the workbook therefore uses leukocyte-fraction and composition-sensitive terminology. PET observations at Days 14, 21, and 28 are independent cross-sectional measurements. Figure 7E contains dose-by-time group summaries and does not report a correlation or inferential test. Transcriptomic, behavioral, and imaging data are presented as parallel study components. Version 2.0.0 aligns the files with the final revised manuscript, including specimen terminology, figure-panel mappings, worksheet and column labels, data dictionaries, software information, derived/statistical source tables, and portable Figure 2–7 scripts. Raw sequencing reads are not included. This standalone release consolidates the final Supplementary Data workbook and Figure 2–7 reproducibility resources for citation with the revised manuscript.