Lymphoid and Myeloid Immune Cell Subsets Gene Markers and their Upregulation after Activation with Enterobacter Cloacae by using the Immune Cell Culture Technique

Abstract

Enterobacter cloacae is a gram-negative, anaerobic bacterium that is capable of causing a variety of infections. Our study is focusing on the inflammatory role of Enterobacter cloacae across different immune cell cultures in vitro, including splenocytes and lymph nodes. The results of our study suggested a pro-inflammatory role of Enterobacter cloacae, inducing inflammatory gene markers of interest after cultivation with immune cells that were isolated from experimental animals(mice), mimicking the environmental human body conditions by using the immune cell culture technique. In our study, the fold change of proinflammatory gene markers of T lymphocytes, B lymphocytes, myeloid lineage and IL6 was investigated compared with the control group (inactivated immune cells group). Interestingly, the results of qPCR indicated a severe activation of all proinflammatory markers in gene expression, including CD3, CD19, GMCSF and IL6 in the activated group compared with the non-activated group. All in all, our results suggested the pro-inflammatory role of Enterobacter cloacae activated Lymphoid lineage and myeloid lineage, where these markers have been investigated in vitro by using immune cell culture technique.