It is revealed that a wide variety of mutation types are possible with the base editors in this VLP collection, revealing that a wide variety of mutation types are possible with the base editors in this collection.
Asfar Lathif Salaudeen, Trevor Shyiak, Carl G. de Boer· bioRxiv· 0 citations
An efficient Cas9d system (Cas9dUltra) is developed through gRNA and protein engineering, and its base editors (9dBEs) further developed through gRNA and protein engineering, enabling efficient and precise genome editing in human cells.
Qingquan Xiao, Zhijin Tian, Luqi Weng et al.· Advancement of science· 0 citations
The effectiveness of the SaCas9 modular base editors, the robustness of the platform’s modularity, and its feasibility for convenient screening of target-specific base editors are demonstrated.
J. Collantes, Kellen Xu, M. Ruiz-Urigüen et al.· The CRISPR Journal· 0 citations
This review focuses on the action mode of different base editors, highlights their interplays with the TLS, summarizes their potential therapeutic applications and discusses perspective strategies to improve precision and expand targeting scope.
Rui Tao, Min Li, Tongyun Luo et al.· Biotechnology Advances· 0 citations
A single-nucleotide resolution ABE created by fusing a nickase Cas9, nCas9-H840A, with the deaminase domain of ADAR, which acts on DNA:RNA hybrids, instead of TadA, which acts on single-stranded DNA in conventional ABEs.
Hyeon Woo Im, Bada Jeong, Yeji Lee et al.· Nature Biotechnology· 1 citation
Key remaining challenges include bystander editing within the activity window, residual off-target DNA and RNA editing, delivery constraints, delivery constraints, immunogenicity, and the need for durable long-term safety evidence across relevant cell types and disease contexts.
Moksada Regmi, K. Ma, C. Bi et al.· Cell Genomics· 0 citations