Spatial and Multi-Omics Analysis of Human Breast Cancer Reveals the Spatiotemporal Dynamics of Basal Layer Disruption

Deciphering the association of spatial landscapes of DCIS with recurrence as invasive breast cancer

Stromal cell composition has been proposed as a potential biomarker in ductal carcinoma in situ (DCIS) for assessing the risk of subsequent recurrence or progression to invasive breast cancer (IBC). However, the characteristics of the tumor microenvironment within DCIS associated with these outcomes remain poorly under...

Abstract PR007: Integrated spatial proteomics of human PDAC uncovers an expanded tumour-immune-stroma spectrum with genomic associations

Pancreatic ductal adenocarcinoma (PDAC) exhibits extensive cellular and molecular heterogeneity. How unique malignant states interact with and organize local microenvironments to drive therapy resistance and progression remains poorly understood, limiting tumour profiling and patient stratification. Existing binary t...

Spatial transcriptomics reveals site-specific cellular and metabolic heterogeneity in bladder carcinoma in situ

Results demonstrate that CIS exhibits significant intra-patient heterogeneity not captured by single-site profiling, and support multi-region sampling, which could help improve risk stratification, biomarker development, and prediction of response to intravesical therapies.

ctDNA Release Heterogeneity in Luminal Breast Cancer Revealed by Single-Cell Multi-Omics Spatial Analysis

Backgroud Breast cancer is a leading cause of cancer-related mortality, and HR+/HER2− is the predominant subtype. Molecular pathology, including PIK3CA and ESR1 mutation, informs prognosis and treatment selection. Circulating tumor DNA (ctDNA) offers a non-invasive approach for cancer monitoring, but its clinical utili...

Spatial transcriptomics reveals atorvastatin modulates the tumor microenvironment to suppress breast cancer liver metastatic outgrowth

Metastatic breast cancer remains incurable, underscoring the need for novel therapeutic strategies. Epidemiological and preclinical evidence suggests statins suppress metastatic outgrowth, however the underlying mechanisms remain unclear. We employed high-resolution spatial transcriptomics (ST) to explore the cellular...

Spatial analysis of head and neck cancer identifies two ecosystems with distinct modes of epithelial-to-mesenchymal transition