CONSTRUCTION OF CANDIDA FAMATA STRAINS WITH ENHANCED TOLERANCE TO LIGNOCELLULOSIC HYDROLYSATE INHIBITORS

Abstract

Lignocellulosic biomass is a promising source of sugars, however, during hydrolysis the toxic compounds are released, that can inhibit the producing strain. For today there is no data on the genetic regulation in C. famata of tolerance to inhibitors (furfural, hydroxymethylfurfural (HMF), and acetic acid), as well as whether increased resistance would correlate with increased riboflavin production. Overexpression of ADH6 (coding for alcohol dehydrogenase), HAA1 (coding for transcription factor), and GRE2 (coding for aldehyde reductase) genes were performed for C. famata BRPI strain. Resulted strains were characterized by increased in riboflavin production. Keywords: riboflavin; Candida famata; yeast; riboflavin overproducers; lignocellulose hydrolysate; inhibitors.