Oct 2026· Journal of Agricultural and Food Chemistry· Vol 74 41, pp.
32514-32526
· 0 citations· 34 references
Medicine
TL;DR
An edible lactic acid bacteria (LAB) delivery system is developed, constructing a recombinant strain expressing interferon-γ (IFN-γ) via the nisin-inducible (NICE) system for active IFN-γ release, aiming to provide a novel therapeutic with both probiotic and antiviral functions.
Abstract
Viral enteritis severely impacts the animal industry. Current vaccines and antibiotics have limited efficacy due to virus mutation, drug resistance, and side effects. This study developed an edible lactic acid bacteria (LAB) delivery system, constructing a recombinant strain (pNZ8149-IFN-γ/NZ3900) expressing interferon-γ (IFN-γ) via the nisin-inducible (NICE) system for active IFN-γ release, aiming to provide a novel therapeutic with both probiotic and antiviral functions. Using a mouse viral enteritis model and intestinal organoid coculture, the strain was shown to significantly alleviate intestinal inflammation and tissue damage and reduce serum inflammatory factors. An acute virulence test confirmed its safety. Furthermore, at the organoid level, the recombinant LAB resisted Poly(I:C)-induced inflammation and damage. The innovation lies in the first combination of IFN-γ with food-grade LAB for oral delivery and targeted immunomodulation, offering a new antibiotic-free strategy for preventing and treating viral enteritis.
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Acute viral hepatitis is characterized by excessive inflammatory and antiviral immune responses that contribute to liver injury. Polyinosinic-polycytidylic acid [poly(I:C)], a synthetic analog of viral double-stranded RNA, is widely used to mimic virus-induced acute liver damage. Increasing evidence indicates that immu...
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Calves’ immature immune systems make them susceptible to various infections. In particular, diarrhea and respiratory diseases impair growth and increase mortality, resulting in substantial economic losses in the cattle industry. Probiotics are living microorganisms that confer health benefits to the host and are increa...
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