Two edgeR-based pipelines were constructed that would allow for direct comparison between two genome-wide CRISPR screening datasets of colorectal carcinoma, the Wellcome Sanger Institute’s (Sanger) and the Broad Institute's DepMap (Achilles) datasets, and Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK) for comparison and validation.
This chapter outlines a comprehensive methodology for the design, assembly, and functional assessment of CRISPR/dCas9 systems optimized for tomato to investigate pathogen-associated responses.
Ananya Mukherjee, Shrabani Basak, Raghuvir Singh et al.· Methods in molecular biology· 0 citations
Examination of the predicted secondary structure of the tracrRNA–crRNA duplex suggests that the features required for Cas9-catalyzed DNA cleavage at specific sites can be captured within a single chimeric RNA.
A. Udristioiu, Manole Cojocaru· Clinical Cancer Research· 0 citations
Examination of the predicted secondary structure of the tracrRNA–crRNA duplex suggests that the features required for Cas9-catalyzed DNA cleavage at specific sites can be captured within a single chimeric RNA.
A. Udristioiu, Manole Cojocaru· Clinical Cancer Research· 0 citations
Examination of the predicted secondary structure of the tracrRNA–crRNA duplex suggests that the features required for Cas9-catalyzed DNA cleavage at specific sites can be captured within a single chimeric RNA.
A. Udristioiu, Manole Cojocaru· Clinical Cancer Research· 0 citations
Examination of the predicted secondary structure of the tracrRNA–crRNA duplex suggests that the features required for Cas9-catalyzed DNA cleavage at specific sites can be captured within a single chimeric RNA.
A. Udristioiu, Manole Cojocaru· Cancer Research· 0 citations
It is reported that the choice of sgRNA target site affects its targeted cleavage activity and show that the combination of guideRNA with SpCas9 can effectively delete the exonic fragment of the SLC25A38 gene in HEK293T cells.
S. Yazdanparast, Hamid Galehdari, S. Khatami et al.· Journal of Applied Genetics· 0 citations