POSTBIOTIC WOUND-HEALING COMPOSITION BASED ON METABOLITES OF PROBIOTIC BACILLUS VELEZENSIS STRAINS

Abstract

Introduction. The issue of developing and introducing new, effective probiotics into medical and veterinary practice is of great relevance. This is linked to the increasing frequency of microbiome imbalances, which have a negative impact on the body’s health, as well as to the circulation of large numbers of microbial strains that are multi-drug resistant to antibacterial agents in the environment. Probiotics are increasingly being regarded as an alternative to traditional and potentially harmful antibiotic chemical preparations, in particular antibiotics. Today, there is a need to develop probiotic preparations based on beneficial microorganisms and their metabolites for the prevention and treatment of various pathological conditions in humans and animals, particularly for the prevention and treatment of infected wounds. Aim. The aim of this study was to develop a complex postbiotic wound-healing composition based on cell-free supernatants of the probiotic strains Bacillus velezensis IMV B-7142 and IMV B-7143, cultured in the presence of cerium dioxide nanoparticles, and to investigate its therapeutic properties in vivo. Methods. The therapeutic efficacy of the developed postbiotic composition was investigated in male Chinchilla breed rabbits in in vivo model experiments involving mono- and mixed wound infections with test pathogen cultures. Results. A comprehensive wound-healing composition has been developed based on cell-free supernatants of the probiotic strains B. velezensis IMV B-7142 and IMV B-7143, cultured in the presence of cerium dioxide nanoparticles (nano-CeO₂) at a concentration of 0.01 mM. In model studies on Chinchilla rabbits, it was shown that topical application of the postbiotic formulation accelerated wound clearance and tissue regeneration, reducing the time to complete healing of purulent wounds from 24 days (in the control group) to 14–18 days (in the experimental group), depending on the infectious agent. Conclusions. The research results indicate the high therapeutic potential of the developed postbiotic composition and the promise of its application as a safe alternative approach to the treatment of skin wound disorders and wound infections. The priority of this development is confirmed by a Ukrainian patent for a utility model.