Indole-3-Acetic Acid Production by Klebsiella variicola from Watermeal and Its Role in Enhancing Watermeal Growth

Abstract

Plant growth-promoting bacteria (PGPB) enhance plant development through mechanisms such as the production of indole-3-acetic acid (IAA). However, endophytic bacteria associated with aquatic plants, particularly watermeal (Wolffia spp.), remain poorly explored. This study investigated the potential of bacterially derived crude IAA extract for enhancing watermeal growth and accumulation of secondary metabolites in watermeal. This study isolated and screened endophytic bacteria from watermeal for IAA production, identifying Klebsiella variicola W1/6 as the most efficient producer. Optimization using response surface methodology (RSM) revealed optimal conditions at pH 5.84, 5 days of incubation, and 10.63% (v/v) inoculum size. Crude IAA extract significantly enhanced watermeal growth under both laboratory and greenhouse conditions. Notably, the combination of 0.5 ppm crude IAA extract with 50% chemical fertilizer produced growth comparable to that of the full fertilizer treatment, indicating reduced fertilizer requirement. These findings highlight the potential of bacterially derived IAA from K. variicola W1/6 as a microbial biostimulant for sustainable aquatic plant cultivation.