Jul 2026· ACS Bio & Med Chem Au· Vol 6, pp. 323 - 328· 0 citations· 27 references
Medicine
Abstract
The variable domains of heavy chain only antibodies, also called nanobodies, have provided powerful new tools for biomedical research. Their rapid development across multiple fields has helped address emerging scientific and clinical challenges, establishing single-domain antibodies (sdAbs) as highly adaptable platforms for a wide range of biotechnological and therapeutic applications. In this study, we identified a nanobody from an alpaca immune library, sdAbCM1, that binds to the maltose-maltodextrin binding protein, MBP, with picomolar affinity. Biophysical investigations showed that sdAbCM1 recognizes a yet unreported epitope and interacts with MBP in its closed conformation when maltose is present. The high affinity, epitope specificity, and ability to tolerate the ligand-bound state render sdAbCM1 a valuable and versatile biotechnological tool with potential applications such as detection, localization, and purification of MBP fusion proteins and protein complex engineering for single-particle microscopy.
A general protocol for the molecular grafting of two peptide affinity motifs onto OmpG, followed by purification and characterization of the engineered construct, to generate a single-channel multiplex sensor is presented.
Joshua C. Foster, Min Chen· Methods in molecular biology· 0 citations
Traditional in vitro screening of a nanobody library combined with an intracellular bioluminescence resonant energy transfer (BRET) proximity sensor approach for sequence down-selection can yield strong in vitro binders that also perform well as intrabodies, in this case capable of binding to and inhibiting the enzymat...
J. Wang, Victor L. Lam, E. Dunn et al.· bioRxiv· 0 citations
A novel nanobody-fusion protein platform was established that functioned as high-affinity binders for target antigens and heat-stable signal tracers for immunoassay detection and quantitative analysis, providing a novel type of thermostable immunoreagent.
Hong-Heng Li, Chen-Chen Zhou, Xiao-Mei Zhang et al.· Experimental and Therapeutic...· 0 citations
A protocol for discovering protein‐binding peptides using a very large, target‐agnostic yeast surface display library containing approximately 6.1 × 109 unique clones and providing broad coverage of short peptide sequence space is described.
J. D. Hurley, Andrew C. Kruse· Current Protocols· 0 citations
The key finding is that the reliable and time-tested resins like those based on protein A continue to dominate the market, although future trends also point toward smaller, more stable, and cost-effective synthetic ligands for specific applications.
L. N. Ikryannikova, M. N. Tereshin, Milena V. Baskova et al.· International Journal of Mol...· 0 citations
Nanobodies have recently emerged as an alternative to classical antibodies in therapeutic and diagnostic contexts, promising improved stability and simpler manufacturing. However, many labs still rely on low throughput conventional screening methods for nanobody discovery. Here we report streamlined experimental...
William L. White, Edward H. Moseley, Jacqueline M. Tremblay et al.· Journal of Immunology· 0 citations
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