A high-quality chromosome-level genome assembly was generated by integrating MGI DNBSEQ-T7 short-read, PacBio Sequel II long-read, and Hi-C scaffolding technologies to facilitate research on adaptive evolution in Heteroptera.
Abstract
Corizus hyoscyami (Heteroptera: Rhopalidae) is a widely distributed and highly polyphagous insect, making it a relevant species for understanding the evolution of herbivory and host adaptation in true bugs. However, the genome of Corizus hyoscyami has not yet been sequenced, limiting investigations into its biological characteristics. In this study, a high-quality chromosome-level genome assembly was generated by integrating MGI DNBSEQ-T7 short-read, PacBio Sequel II long-read, and Hi-C scaffolding technologies. The assembly was assessed to be 97.6% complete by BUSCO. The final genome was assembled into a size of 1.47 Gb, with a contig N50 of 1.61 Mb and a scaffold N50 of 198.34 Mb. The GC content was determined to be 34%, and 94.90% of the sequences were anchored onto 6 chromosome-level scaffolds, representing the major chromosomal elements of the genome. Repetitive sequences accounted for 69.79% of the genome, and 18,540 protein-coding genes were annotated. This genome assembly is expected to facilitate research on adaptive evolution in Heteroptera.
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