Jul 2026· Journal of the College of Basic Education· 0 citations
TL;DR
Among the 20 isolates, one isolate was identified as Brevibacillus parabrevis PS12, representing the first report from Iraqi soil, which contributed to an increase in bacterial biomass which led to a rise in the specific activity of arginase.
Abstract
Soil-occupy Brevibacillus parabrevius PS12 species are known for their metabolically versatile and it is being discovered as source of natural enzymes valuable to industry. Arginase catalyzes the processing of L-arginine to L-ornithine and urea, it is the one that plays a significant role in nitrogen metabolism Finding novel bacterial strains from environmental sources that are capable of producing the arginase enzyme is crucial for expanding microbial resources for industrial and pharmaceutical use. Among the 20 isolates, one isolate was identified as Brevibacillus parabrevis PS12 (97% 16S rRNA similarity, GenBank accession no. (PX118439.1), representing the first report from Iraqi soil. Arginase activity reached 8.3 U/mg protein a peak in nutritional source with maltose as a arbone source and casein as a Nitrogen source, the physical parameters also effect on enzyme specific activity at 7.0 pH, 37°C, and 48 hours of incubation time with shaker incubator 150 rpm to ensure the homogeneity of all medium components and continuous aeration all these factors contributed to an increase in bacterial biomass which led to a rise in the specific activity of arginase.
L-Asparaginase is an important enzyme with therapeutic applications. Current commercial enzymes from E. coli and Erwinia chrysanthemi have limitations, such as high glutaminase activity, creating a need for more stable alternatives. This study aimed to isolate and optimize the production of L-Asparaginase from native, halotolerant Bacillus subtilis strains (DAR and D6A) isolated from central Iran, to find an alternative source for this therapeutically important enzyme. The enzyme was initially isolated using polyethylene glycol (PEG) precipitation. Key factors affecting enzyme production were screened using the Plackett-Burman design and subsequently optimized via the Box-Behnken response surface methodology. PEG precipitation at concentrations of 15-20% for PEG 4000/6000 and 10-15% for PEG 8000/10000 was most effective for initial enzyme separation, yielding specific activities of 4.8 U/mg and 5.2 U/mg for strains DAR and D6A, respectively. Optimization revealed that the most influential factors for maximum L-Asparaginase production were 17.6 g/L NaCl, 4.99 g/L KH2PO4, and 10 g/L asparagine for strain DAR, and 1.49 g/L glucose, 10 g/L NaCl, and 9.98 g/L asparagine for strain D6A. The isolated halotolerant B. subtilis strains are capable of producing L-Asparaginase. PEG precipitation is a suitable method for initial purification, and statistical optimization successfully identified the critical culture conditions to enhance enzyme yield significantly. These strains represent promising sources for the production of this clinically valuable enzyme.
K. Hosseini, Parvin Akbarzadehlaleh, S. Hallaj-Nezhadi et al.· Preparative Biochemistry & B...· 0 citations
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Overall, UzMU25 exhibited preliminary enzymatic, antagonistic, and biofilm-forming characteristics of biotechnological interest, but whole-genome antimicrobial-resistance screening and more comprehensive phenotypic and toxicological evaluations are required before the strain can be recommended for probiotic or other practical applications.
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Bacterioruberin is a C50 carotenoid gaining more interest because of its important biological properties. This study aimed to the identification and characterization of a bacterial strain producing this attracting carotenoid, Arthrobacter sp. strain CP30. Phylogenetic analysis based on 16S rRNA gene sequences and whole genome comparison showed that strain CP30 belongs to the Arthrobacter agilis group with Arthrobacter bussei being the closest relative. Sequence similarity of the 16S rRNA gene of CP30 to described Arthrobacter species as well as average nucleotide identity values indicate that CP30 represents a new species within the genus Arthrobacter. The strain has been cultivated under controlled fermentation parameters, and the obtained biomass was investigated for carotenoids and lipids quali-quantitative profiles characterization. The biomass extracts enriched in bacterioruberin have been evaluated for antimicrobial and antioxidant properties. The bacterioruberin produced by the strain reached up a carotenoid titer of 1.28 mg L-1 in 72 h, with a yield of 327.67 ± 6.6 μg g-1 of dry biomass, a productivity of 0.02 mg/L/h, and showed an IC50 value of 3.87 μg/mL. The most abundant fatty acids were 12-methyltetradecanoic, 14-methylpentadecanoic and 13-methyltetradecanoic. Whereas, among the intact lipids, glycolipids, phospholipids, diacylglycerols, and triacylglycerols have been identified in the investigated strain. This study allowed pointing out the potential of strain CP30 as a promising bacterioruberin producer, representing an interesting natural carotenoids source to be further investigated as suitable candidate for feed and food application. Comparison of key protein sequences involved in bacterioruberin biosynthesis of CP30 and A. bussei showed sequence similarities of >90%.
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Based on further phenotypic, genomic, and phylogenetic analysis, strain F2T represents a novel genus and species within the family Anaerovoracaceae with the proposed name Peptonella octanoica gen. sp.
Dinesh Kumar Nallasamy, B. Lindner, Christopher E. Lawson· bioRxiv· 0 citations
