Mechanism of Thermosonication in Inhibiting Spore of Heat-Resistant Bacillus subtilis via Metabolic Networks and Gene Regulation

Temperature dependence of Bacillus cereus spores: effects on sporulation, germination behavior, and inner membrane protein responses.

Temperature-dependent variations in the molecular characteristics of intact spores, including changes associated with Ca2+-dipicolinic acid, protein-related, and nucleic acid-related spectral features under different sporulation temperatures are revealed.

Functional role of the diadenylate cyclase gene cdaA in stress responses of the dairy starter Streptococcus thermophilus.

Results showed that cdaA is critical for osmotic and ethanol tolerance but negatively modulates bile salt resistance in S. thermophilus, suggesting that absence of cdaA confers a survival advantage under bile salt stress.

Transcriptomic Analysis Reveals the Time-Dependent Mechanism of Antifungal Activity in Bacillus velezensis GHZJ-1

The results suggest that facing continuously increased environmental stress over time, GHZJ-1 undergoes global transcriptional reprogramming and resource reallocation, downregulating basal metabolism to construct a synergistic antagonistic system coupling chemical defense with nutritional competition.

Trehalose-6-phosphate synthase promotes thermotolerance by governing glycolytic flux in Cryptococcus deneoformans

This study uncovers a previously unappreciated mechanism of Tps1-mediated heat adaptation in C. deneoformans, by revealing that Tps1 functions as a critical metabolic gatekeeper that safeguards glycolytic flux to sustain growth at elevated temperatures.

Stage V sporulation protein G as a global regulator of biofilm formation and virulence in Staphylococcus species: molecular mechanisms and therapeutic potential

A Bacillus thuringiensis ΔcdsR-based expression system for insecticidal proteins identified using AlphaFold3.

Environmentally friendly biopesticides, such as those based on the bacterium Bacillus thuringiensis (Bt), have an important role to play in Integrated Pest Management systems. An increasingly diverse range of pesticidal proteins is being discovered, led by advances in genome sequencing and the bioinformatic identification of candidate genes. In this work, we utilized an AlphaFold3-based structure-prediction approach to identify candidate genes and, in parallel, developed a Bt chassis strain that can be universally used to express new pesticidal proteins. This chassis strain is based on a cdsR deletion strain with a controllable cell death pathway that decouples toxin production from sporulation, enabling cytoplasmic hyperexpression via strong promoters and encapsulation of recombinant proteins. These characteristics provide superior efficacy, UV stability, and environmental safety compared with conventional Bt formulations. Several of the identified proteins displayed insecticidal activity when expressed in this strain, providing a versatile framework for next-generation biocontrol and sustainable pest-management innovation.