Research on the Production of Single-Cell Protein from Gibberellin Fermentation Waste Liquid Using Microbial Methods

Abstract

Efficient monitoring and intelligent regulation of industrial bioprocesses are increasingly important for sustainable resource utilization and advanced manufacturing systems. This study investigates the production of single-cell protein (SCP) from gibberellin fermentation waste liquid using the unconventional yeast Nectaromyces rattus, aiming to achieve simultaneous pollutant removal and biomass valorization. Bottle culture experiments and tank reactor simulations were conducted to evaluate the effects of wastewater dilution ratio and glucose supplementation on microbial growth, nitrogen assimilation, and SCP production. The results indicate that under a 12-fold wastewater dilution with 10 g/L glucose addition, corresponding to an optimized C/N ratio of approximately 18:1, the dry cell weight reaches 192.336 g/L and the ammonia nitrogen removal efficiency attains 85.54%. Furthermore, systematic analyses of strain adaptation, fermentation optimization, and quality evaluation demonstrate the feasibility of integrating biological treatment with high-value protein production for industrial wastewater recycling. The proposed strategy establishes an effective framework for sustainable biomanufacturing and process intensification. In addition, its reliance on real-time reactor monitoring, multiparameter process control, and intelligent optimization provides valuable engineering references for electromagnetic sensing technologies, wireless monitoring architectures, antennaassisted industrial instrumentation, and advanced signal acquisition systems used in next-generation bioprocess automation.