This work analyzes protocol-dependent effects on the colloidal characterization and drug loading/release analysis of model thermosensitive PNIPAM-co-COOH microgels and shows how they can be quantified or minimized through targeted methodological refinements. Findings reveal that standard single-beam DLS underestimates the collapsed hydrodynamic radius by 18% at 43 °C due to thermal convection. After drift correction, 3D-DLS combined with SLS provides a consistent description of thermally induced collapse, pH-dependent swelling and core–corona structure. Regarding drug delivery, loading efficiency for Doxorubicin and 5-Fluorouracil is maximized near the volume phase transition temperature, where hydrophobic interactions are strongest. For release studies, dialysis is recommended, but free-drug blanks are required to account for membrane-induced delay and ensure accurate early kinetic profiles. By integrating TEM, AFM, SLS, DLS, NTA and LDE, this study establishes a robust framework for the colloidal characterization of thermosensitive microgels. These refinements reduce experimental bias and may be extended to related soft nanocarriers.
The solid dispersion of ABA with HPMCAS-LF significantly improved solubility, dissolution, and diffusion, highlighting its potential for enhanced oral bioavailability and improved therapeutic outcomes.
Namrata S Desai, Amol S. Shete, Snehal S. Patil et al.· Drug Development and Industr...· 0 citations
Overall, the study demonstrated that formulation parameters significantly affect drug release, and F4 was identified as the optimal batch for achieving effective sustained delivery of Eperisone hydrochloride.
Padekar Chetana A, Vivek Daniel· International Journal of Dru...· 0 citations
It is demonstrated that apo-Fn undergoes controlled disassembly under mild acidic conditions, and efficiently reassembles upon neutralization or urea removal, and efficiently reassembles upon neutralization or urea removal.
Jiaoqian Shang, Jie Li, Yabo Wei et al.· Biomaterials Advances· 0 citations
Cisplatin-loaded biopolymeric nanogels are promising carriers for sustained anticancer drug delivery; however, establishing physically interpretable links between formulation structure, transport behavior, and temperature-dependent release remains challenging. Here, mucilage-alginate-coated chitosan (MACC) nanogels were developed and evaluated using an integrated framework combining empirical kinetic analysis, mechanistic thermo-diffusive modeling, and complementary interfacial characterization. Basil seed mucilage was incorporated as a hydrophilic shell modifier to tune nanogel physicochemical behavior and release performance. The optimized MACC₂ formulation showed stable core-shell morphology, an average particle size of 75 ± 12 nm, and an encapsulation efficiency of 46.85%. In vitro assays demonstrated high compatibility with normal fibroblast cells and dose-dependent inhibition of MCF-7 breast cancer cells, supporting controlled cisplatin delivery. Drug release in PBS (pH 7.4) followed a biphasic profile with an initial burst stage and a sustained diffusion-dominated regime. Temperature-dependent studies at 35-39 °C showed accelerated release, with effective diffusion coefficients increasing from 2.0 × 10-21 to 3.6 × 10-21 m2 s-1, while Arrhenius analysis supported thermally activated transport within the hydrated polymeric matrix. Empirical kinetic modeling further indicated predominantly diffusion-controlled release with secondary polymer-relaxation contributions. Air-water interfacial tensiometry showed reduced interfacial activity for mucilage-containing nanogels, consistent with increased aqueous affinity and formulation-dependent physicochemical behavior. These measurements were interpreted as complementary descriptors rather than direct evidence of hydration or bulk diffusion. Overall, this integrated empirical-mechanistic-interfacial framework provides a physically interpretable approach for analyzing thermo-diffusive cisplatin transport in hydrated biopolymeric nanogels.
M. Lotfi, Mojtaba Shafiee, A. Sharipova et al.· Colloids and Surfaces B: Bio...· 0 citations
The developed thermosensitive in-situ nanoemulsion gel can be a candidate for presenting an innovative and effective platform for the prolonged topical delivery of lidocaine and prilocaine with favorable physicochemical and stability properties.
Ardeshir Zomorodi, M. Khoshkam, Hoda Nassira· Journal of Pharmaceutical In...· 0 citations
This work reports an integrated experimental and theoretical study of polymeric nanogels as tunable colloidal platforms for selenite binding and controlled ion delivery. Three formulations (N600, N3000, and Nmix) form stable colloidal dispersions (120–260 nm) with structure and interfacial properties governed by polymer architecture and network composition. Performance is evaluated under foliar application in Eruca sativa as a proof-of-concept system, showing enhanced selenium uptake and reduced phytotoxicity compared to free selenite. N600 exhibits the most balanced behavior, combining moderate binding strength, efficient ion release, and high compatibility. Multitechnique characterization and density functional theory (DFT) calculations reveal that selenite interaction with the polymer network is governed by coupled polymer–ion interactions, proton-transfer equilibria, and solvation effects. At pH 5.5, where HSeO3– predominates, binding is dynamic and reversible, enabling polymer-mediated selenium delivery. N3000 and Nmix show stronger stabilization via proton-transfer-assisted interactions, whereas N600 is dominated by weaker electrostatic association, consistent with polymer protonation state and pKa-dependent behavior. The results demonstrate that the balance between ion stabilization and mobility governs binding and release behavior. This study provides molecular-level insight into polymer–ion systems and establishes design principles for tunable colloidal platforms for controlled ion delivery.
H. Andrada, Di Cai, Cristian Huck Iriart et al.· ACS Applied Polymer Material...· 0 citations