Adaptive Evolution in Aminoacyl-tRNA Synthetases Drives Antibiotic Tolerance and Resistance in Clinical Klebsiella pneumoniae Isolates.

Mapping Essential Genes in Klebsiella pneumoniae Using Transposon-Insertion Sequencing (Tn-seq)

The findings may inform the identification of potential therapeutic targets for multidrug-resistant K. pneumoniae and the identification of genes essential for growth on laboratory media.

Transposon mutagenesis uncovers the genetic landscape of streptomycin susceptibility and implicates SbmA in aminoglycoside uptake in Escherichia coli

Broad adaptations of plasmid-carrying Escherichia coli and Klebsiella pneumoniae to water as a reservoir

Findings demonstrate strain-specific adaptive responses to aquatic environments and underline the context-dependent influence of plasmids in shaping adaptation.

Genomic and structural analysis of dacB variants associated with cephalosporin resistance in Pseudomonas aeruginosa

Structural insight is offered of dacB variants leading to antibiotic resistance and the need to prioritize specific antibiotics to address the challenges arising from protein mutations is emphasized.

Emergence of mutHV76G in carbapenem-resistant Klebsiella pneumoniae disrupts DNA mismatch repair and results in a hypermutator phenotype

It is confirmed that the mutH mutation accelerates resistance development and increases the bacterium’s ability to acquire resistance plasmids, and the same types of mutations that appeared under laboratory conditions also emerged during infection.

Global lessons from antibiotic resistance: metformin-hydrolyzing genes in transposable elements, a new threat for type II diabetic patients?

Metformin pollution appears to promote the emergence and mobilization of pharmaceutical-degrading genes through mechanisms paralleling antibiotic resistance evolution, and these findings reveal a dynamic modular assembly of metabolic functions facilitating adaptation to pharmaceutical pollutants.