Association of Arg16Gly and Gln27Glu Polymorphic Variants of the β2-Adrenergic Receptor Gene with Liver Cirrhosis
TL;DR
Haplotype Arg16ArgGln27Gln of β2-AR gene is a predictor of LC development, increasing the risk of disease development 2,4 times and is associated with LC severity according to Child-Pugh classification.
Abstract
Purpose of the study. To evaluate the interaction of polymorphic variants rs1042713 (Arg16Gly) and rs1042714 (Gln27Glu) of the β2-adrenoreceptor (β2-AR) gene with the development of liver cirrhosis (LC). Materials and methods. A total of 137 patients with liver cirrhosis and 143 healthy volunteers, who were Caucasian and unrelated to each other, participated in the observational case-control study. Genotyping of polymorphic variants Arg16Gly and Gln27Glu of the β2-AR gene was performed using the polymerase chain reaction (PCR) method by analyzing restriction fragment length polymorphism of amplicons (PDRF analysis). Results. Genotypes AG, GG and AA of the Arg16Gly polymorphism and genotypes CG, CC and GG of the Gln27Glu polymorphism of the β2-AR gene are not predictors of LC development (OR = 0.86; 95% CI: 0.53-1.37; p=0.52; OR=0.88; 95% CI:0.55-1.41; p=0.59; OR=1.81; 95% CI:0.91-3.62; p=0.09; OR=0.74; 95% CI:0.46-1.19; p=0.22; OR=1.55; 95% CI:0.93-2.58; p=0.09; OR=0.91; 95% CI: 0.52-1.57, p=0.72, respectively, p>0.05). Carrying the Arg16Arg/Gln27Gln haplotype increased the chance of developing LC by 2,4-fold (OR=2.42; 95%DI:1.13-5.18; p=0.02). Arg16ArgGln27Gln haplotype is associated with LC severity according to Child-Pugh classification (χ2=3.27; p=0.007) and severity of liver fibrosis according to APRI index (χ2=4.46; p=0.03). Conclusion. Haplotype Arg16ArgGln27Gln of β2-AR gene is a predictor of LC development, increasing the risk of disease development 2,4 times.