The prokaryotic expression system was used to express the recombinant BCoV N protein and used it as an immunogen to immunize BALB/c mice and showed that the mAb 4D8 can identify the epitope spanning and research results showed that the mAb 4D8 can identify the epitope spanning.
This work clarifies the molecular basis of PRV neutralization and identifies a conserved epitope, which upon further validation could serve as a diagnostic candidate and inform antiviral research.
Yu-Mei Chen, Jie Gao, Rui-Rui Liu et al.· Archives of Microbiology· 0 citations
This study identifies a novel, highly conserved with conservative substitutions linear B-cell epitope on the RABV glycoprotein, providing a precise molecular target for the rational design of next-generation epitope-based vaccines and specific diagnostic tools.
Yu-Mei Chen, Xiao-Hui Zhang, Yan-Hua Qi et al.· Medical Microbiology and Imm...· 0 citations
Findings support the feasibility of the multi-epitope construct and provide a foundation for future investigations of its immunogenicity and protective efficacy studies in animal models.
Nadálin Yandra Botton, Guilherme Feijó de Sousa, Marina Sturbelle Garcia et al.· Journal of Biotechnology· 0 citations
Epizootic hemorrhagic disease virus (EHDV) is an important Orbivirus transmitted by culicoides midges. EHDV poses a significant threat to ruminant production worldwide. The VP7 protein is a highly conserved, group-specific antigen of EHDV, which serves as a key target for serological diagnosis. In this study, the recom...
Xin-Bing Hu, Yun-Ru Zhong, Ying-Juan He et al.· Veterinary Research· 0 citations
Avian reovirus (ARV) causes of multisystemic, immunosuppressive infectious diseases in poultry. Among them, the σC protein, a structural component of the outer capsid encoded by the S1 genome segment, plays a crucial role in viral attachment and cellular entry. In this study, we expressed the σC protein derived from th...
A novel linear B-cell epitope, 97LGDGDEIR104, is identified within the flexible N-terminal region of gB, which was critical for antibody binding, revealing a new antigenic site on VZV gB.
Yu-Mei Chen, Meng-Ru Yang, Hong-Liang Liu et al.· Archives of Microbiology· 0 citations
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